Among engineered nanomaterials, CeO₂ nanoparticles (NPs) are of particular interest due to their unique properties. These NPs’ anti-oxidative and auto-reduction natures also render them promising in scavenging reactive oxygen species (ROS). In this study, different synthesis methods of CeO₂ NPs were studied: an NH₄OH precipitation method, a NaOH precipitation method, and a microwave hydrothermal synthesis. Among these methods, the one-pot facile NH₄OH method outperformed the others. The synthesized CeO₂ NPs were determined to be highly monodispersed 13 nm crystallites with a pH at the potential of zero point charge (pH_zpc) of 2.2. The valence states of Ce in CeO₂ NPs were 3 + and 4 + were determined. The synthesized CeO₂ NPs remained stable over 2 days in a medium containing FBS and over 3 days in NaCl solutions. The auto-reduction oxidation cycles of our CeO₂ NPs were able to scavenge ROS multiple times. The cytotoxicity of NPs showed CeO₂ NPs did not have a significant toxic effect on neuro2A cells. Owing to its synthesis of NPs of small size, with anti-oxidative/auto-reduction nature, with stability in cell culture media, and an absence of cytotoxicity toward neuroblastoma cells, this novel method proves promising as a method to generate CeO₂ NPs for biomedical applications.

在工程纳米材料中，CeO ₂纳米颗粒（NPs）由于其独特的性能而特别受关注。这些NP的抗氧化和自动还原特性也使其有望清除活性氧（ROS）。在这项研究中，研究了CeO ₂ NPs的不同合成方法：NH ₄ OH沉淀法，NaOH沉淀法和微波水热合成。在这些方法中，一锅法NH ₄ OH方法优于其他方法。合成的CeO ₂ NPs被确定为高度单分散的13 nm微晶，其零点电荷电位（pH _zpc）的pH值为2.2。CeO ₂中Ce的价态NP分别为3 +和4 +。合成的CeO ₂ NP在含有FBS的培养基中保持2天稳定，在NaCl溶液中保持3天稳定。我们的CeO ₂ NP的自动还原氧化循环能够清除ROS多次。NPs的细胞毒性表明CeO ₂ NPs对Neuro2A细胞没有明显的毒性作用。由于其具有小尺寸，具有抗氧化/自动还原性质，在细胞培养基中稳定并且对神经母细胞瘤细胞无细胞毒性的NP的合成，这种新方法被证明是有希望的一种产生CeO ₂ NPs的方法。生物医学应用。