Abstract

Anopheles mosquitoes became one of the biggest problems threaten human life through causing malaria disease. Hereby, Selenium nanoparticles (Se-NPs) as anti-victor malaria were fabricated for the first time through Penicillium corylophilum in presence of ascorbic acid as a reducing agent. Antibacterial versus pathogenic Gram +Ve and Gram −Ve bacteria, as well, in vitro cytotoxicity against two types of cell lines (normal Wi 38 and cancer Caco-2) were explored prolonging with their larvicidal activity towards Anopheles stephensi mosquitoes. Se-NPs is characterized by UV–Vis spectroscopy, FT-IR, TEM, EDX, XRD, DLS. Results affirmed the ability of Penicillium corylophilum to build up Se-NPs in spherical shape with average size 29.1–48.9 nm. In addition, Se-NPs exhibited broad spectrum activity against pathogenic Gram +Ve and Gram −Ve bacteria, while the results obtained from cytotoxicity evaluation signified clearly that, alteration occurred in the cells was represented as loss of their typical shape, partial or complete loss of monolayer, granulation, shrinking or cell rounding with IC₅₀ value of 171.8 and 104.3 ppm Wi 38 and Caco-2 cell lines respectively, which revealing the high toxicity of Se-NPs towards cancer cells compared with normal cells. However, Se-NPs displayed larvicidal activity against causative malaria vector Anopheles stephensi.

中文翻译：

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绿色合成的硒青霉菌对绿色硒纳米粒子的抗菌，细胞毒性和杀幼虫活性

摘要

按蚊的蚊子成了最大的问题之一，通过导致疟疾疾病威胁人类的生命。据此，在抗坏血酸作为还原剂的存在下，通过嗜青青霉首次制备了作为抗胜利者疟疾的硒纳米颗粒（Se-NPs）。还研究了抗菌与致病性革兰氏+ Ve和革兰氏-Ve细菌对两种细胞系（正常Wi 38和癌症Caco-2）的体外细胞毒性，并延长了它们对斯蒂芬按蚊的杀幼虫活性。Se-NPs的特征在于紫外可见光谱，FT-IR，TEM，EDX，XRD，DLS。结果证实了嗜青霉菌的能力建立球形的硒纳米颗粒，平均粒径为29.1–48.9 nm。另外，Se-NPs对致病性革兰氏+ Ve和革兰氏-Ve细菌表现出广谱活性，而从细胞毒性评估获得的结果清楚地表明，细胞中发生的改变表示为其典型形状的丧失，部分或完全丧失。 IC ₅₀值分别为171.8和104.3 ppm Wi 38和Caco-2细胞系的单层，颗粒化，收缩或细胞变圆，这表明与正常细胞相比，Se-NP对癌细胞具有高毒性。然而，Se-NPs显示出针对致病性疟疾媒介按蚊的杀幼虫活性。