Abstract

Cancer is one of the incurable diseases in the world. Hence, we have effectively synthesized the PEGlyated carbon nanodots loaded cisplatin PEG-CDs@Pt(IV) for targeted therapy of lung cancer therapy. The PEG-CDs@Pt(IV) confirmed by the electroscopic and spectroscopic methods (TEM, DLS, IR and UV–Vis). The In vitro cytotoxicity of the nanoparticles examined two lung cancers (A549 and HEL-299 cells), and one non-cancer cell line (HUVECs) were used in this study by using MTT assay. The nanoparticles are effectively killing cancer cells without affecting the non-cancerous cells. Further we established the morphological changes were of A549 cancer cells, which clearly displays that the nanoparticles induce apoptosis. By compared with PEG-CDs@Pt(IV) and PEG-CDs@Pt(IV) + NIR-NPs, the NIR mediated nanoparticles effectively induce the apoptosis. Also we examined the apoptosis by using flow cytometry, the outcomes suggested that the using PEG-CDs@Pt(IV) + NIR-NPs ultimately killed the A549 cancer cells with IC₅₀ concentrations and were quantified.

中文翻译：

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载有顺铂的近红外光驱动PEG包裹碳纳米点用于肺癌治疗的靶向治疗

摘要

癌症是世界上无法治愈的疾病之一。因此，我们已经有效地合成了载有顺铂PEG-CDs @ Pt（IV）的聚乙二醇化碳纳米点，用于肺癌的靶向治疗。的PEG-CDS @铂（IV）证实了验电和光谱方法（TEM，DLS，IR和UV-VIS）。该纳米颗粒的体外细胞毒性检查了两种肺癌（A549和HEL-299细胞），并且通过MTT分析在本研究中使用了一种非癌细胞系（HUVEC）。纳米颗粒有效杀死癌细胞而不影响非癌细胞。进一步我们确定了A549癌细胞的形态变化，这清楚地表明纳米颗粒诱导了细胞凋亡。与PEG-CDs @ Pt（IV）和PEG-CDs @ Pt（IV）+ NIR-NPs， NIR介导的纳米颗粒可有效诱导细胞凋亡。我们还通过流式细胞术检查了细胞凋亡，结果提示使用PEG-CDs @ Pt（IV）+ NIR-NPs最终以IC ₅₀浓度杀死A549癌细胞并进行了定量。