Hydrogels that mimic the composition and properties of the extracellular matrix have attracted great attention as potential polymeric scaffolds for tissue engineering applications. In this study, an injectable hydrogel composed of hyaluronic acid and gelatin was developed via the oxidative coupling reaction using horseradish peroxidase (HRP). The hydrogel was prepared by mixing two phenol-conjugated polymer solutions, hyaluronic acid-tyramine (HA-TA) and gelatin-hydroxyphenyl propionic acid (GH), in the presence of HRP and hydrogen peroxide (H₂0₂). The gelation rate and mechanical properties of composite hydrogel were controlled by adjusting the HRP and H₂0₂ concentrations, respectively Compared to the pure HA-TA hydrogels, the stability and cellular behaviors of composite hydrogel improved significantly In addition, the injectable hydrogel system showed good performance in 3D printing with high cell viability after one day of printing. The results suggest that enzymatically crosslinked HA-TA and GH hybrid (HA-TA/GH) composite of HA-TA and GH hydrogel has the potential as a material for tissue engineering and 3D printing biofabrication.

中文翻译：

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酶可交联的透明质酸-明胶杂化水凝胶作为组织再生的潜在生物墨水。

模仿细胞外基质的组成和性质的水凝胶作为组织工程应用的潜在聚合物支架备受关注。在这项研究中，由透明质酸和明胶组成的可注射水凝胶是通过辣根过氧化物酶（HRP）的氧化偶联反应开发的。通过在HRP和过氧化氢（H ₂ 0 ₂）存在下混合两种酚共轭聚合物溶液，透明质酸-酪胺（HA-TA）和明胶-羟苯基丙酸（GH）来制备水凝胶。通过调节HRP和H ₂ 0 ₂来控制复合水凝胶的胶凝速率和力学性能。分别与纯HA-TA水凝胶相比，复合水凝胶的稳定性和细胞行为得到了显着改善。此外，可注射水凝胶系统在打印1天后在3D打印中表现出良好的性能，并具有高细胞活力。结果表明，HA-TA和GH水凝胶的酶促交联HA-TA和GH杂化（HA-TA / GH）复合材料具有作为组织工程和3D打印生物制造材料的潜力。