Triticum boeoticum (2n = 2x = 14, A^bA^b) contains beneficial traits for common wheat improvement. The discrimination of A^b-genome chromosomes from A-genome chromosomes is an important step in gene transfer from T. boeoticum to common wheat. In this study, fluorescence in situ hybridization (FISH) analysis using nine oligonucleotide probes revealed high divergence between chromosomes of the common wheat germplasm Crocus and T. boeoticum accession G52. The combination of Oligo-pTa535-HM and Oligo-pSc119.2-HM can differentiate A^b and A chromosomes within homologous groups 2, 4, 5, and 6; chromosomes 2A^b and 6A^b can be identified by using (ACT)₇, (CTT)₇, and (GAA)₇. The probes Oligo-pTa713 and (ACT)₇ can be utilized for the identification of chromosomes 1A^b and 3A^b, respectively. Probes (CAG)₇ and (CAC)₇ can be applied in the identification of 7A^b. Moreover, probe combinations consisting of Oligo-pTa535-HM and (AAC)₇ with (ACT)₇ or (CTT)₇ and of Oligo-pTa535-HM and Oligo-pTa713 with (CAC)₇ or (CTT)₇ will help discriminate the A^b-genome chromosomes of T. boeoticum. These probes are being used as potential markers to select common wheat Crocus-T. boeoticum G52 alien chromosome lines. Moreover, FISH patterns are highly divergent between A^b- and A-genome chromosomes, indicating that obvious chromosome structural variations arose during wheat evolution.

中文翻译：

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使用寡核苷酸探针在 Ab 和 A 基因组染色体之间进行 FISH 核型比较。

Triticum boeoticum (2n = 2x = 14, A ^b A ^b ) 包含对普通小麦改良的有益特性。A ^b基因组染色体与A 基因组染色体的区分是从T. boeoticum到普通小麦的基因转移的重要步骤。在这项研究中，使用九个寡核苷酸探针的荧光原位杂交 (FISH) 分析揭示了普通小麦种质番红花和T. boeoticum种质 G52 的染色体之间的高度差异。Oligo-pTa535-HM 和 Oligo-pSc119.2-HM 的组合可以区分同源组2、4、5和 6 内的A ^b和 A 染色体；染色体 2A ^b和 6A ^b可以通过使用(ACT) ₇、(CTT) ₇和(GAA) ₇来识别。探针 Oligo-pTa713 和 (ACT) ₇可分别用于鉴定染色体 1A ^b和 3A ^b。探针(CAG) ₇和(CAC) ₇可用于鉴定7A ^b。此外，由 Oligo-pTa535-HM 和（AAC）₇与（ACT）₇或（CTT）₇以及 Oligo-pTa535-HM 和 Oligo-pTa713 与（CAC）₇或（CTT）₇组成的探针组合将有助于区分A ^b基因组染色体T. boeoticum。这些探针被用作选择常见小麦番红花-T . boeoticum G52 外来染色体系的潜在标记。此外，A ^b基因组染色体和 A 基因组染色体之间的 FISH 模式高度不同，表明小麦进化过程中出现了明显的染色体结构变异。