Cu(II) anchored polymeric cryogels are synthesized for the isolation of B12. The poly(2-hydroxyethyl methacrylate) [poly(HEMA)] is used as solid support and N-methacryloyl-l-aspartic acid, is used as a ligand. Synthesis success is proved by the Fourier transform infrared spectroscopy, ¹H and ¹³C nuclear magnetic resonance, scanning electron microscope, N₂ adsorption method (Brunauer, Emmet ve Teller), elemental analysis, induction coupled plasma optical emission spectroscopy. In the first part of the work, the polymeric material is synthesized and characterized, and in the second part, adsorptive treatment was carried out to determine the optimum B12 adsorption conditions with this synthesized material in varying conditions. The B12 isolation from cheese is carried out to determine the performance of the polymeric material in the real environment. The antioxidant capacity of the obtained B12 is an indication that the isolation process is successfully carried out.

中文翻译：

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新型用于生化分离的铜改性冰晶的开发

合成了铜（II）锚定的聚合物冰晶，用于分离B12。聚（甲基丙烯酸2-羟乙酯）[聚（HEMA）]用作固体载体，N-甲基丙烯酰基-1-天冬氨酸用作配体。傅里叶变换红外光谱，¹ H和¹³ C核磁共振，扫描电子显微镜，N ₂证明了合成成功。吸附法（Brunauer，Emmet ve Teller），元素分析，感应耦合等离子体发射光谱。在工作的第一部分中，合成并表征了聚合材料，在第二部分中，进行了吸附处理，以确定了该合成材料在不同条件下的最佳B12吸附条件。进行奶酪中的B12隔离以确定聚合物材料在实际环境中的性能。所获得的B12的抗氧化能力表明成功进行了分离过程。