The ultimate goal of organ regenerative therapy is to reproduce fully functional organs to replace which have been damaged as a result of diseases or injury. Although several studies claimed that using different types of cells in some animal models promote hair follicles regeneration, more researches can be done to develop a sufficient and efficient protocol to induce hair generation from different animal models. In this study, we investigated the therapeutic potentials for hair follicle formation by injecting a mixture of epidermal stem cells and dermal papilla cells. Those cells were isolated and culture-expanded. Then we randomly allocated 8 nude mice into two groups. The experiment group received an injection of a mixture that containing of epidermal stem cells and dermal papilla cells. The control group received injection of keratinocyte serum-free medium. The hair follicles regeneration was observed and the injection area was harvested for HE staining. 14 day later, the regenerated hair shafts were observed and HE staining indicated that the newly hair follicle formed the correct structures in experiment group. Furthermore, the mixture injection induced a regular and multilayered stratified epidermis and the epidermis contained of hair follicle-likes structures. Our data showed that injection of a mixture of epidermal stem cells and dermal papilla cells could induce hair follicles regeneration and well-ordered epidermis formation. This study emphasized that the rearrangement of the interactions during seed cells and the niches of the seed cells is essential and necessary for tissue-engineered construct success.

中文翻译：

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裸鼠注射表皮干细胞和真皮乳头细胞促进毛囊再生的初步研究。

器官再生治疗的最终目标是再生出功能齐全的器官，以替代因疾病或受伤而受损的器官。尽管一些研究声称在某些动物模型中使用不同类型的细胞可以促进毛囊再生，但还可以进行更多的研究来开发足够且有效的方案来诱导不同动物模型的毛发生成。在这项研究中，我们通过注射表皮干细胞和真皮乳头细胞的混合物来研究毛囊形成的治疗潜力。这些细胞被分离并培养扩增。然后我们将8只裸鼠随机分为两组。实验组注射含有表皮干细胞和真皮乳头细胞的混合物。对照组注射角质形成细胞无血清培养基。观察毛囊再生情况并采集注射区域进行HE染色。14天后观察再生毛干，HE染色显示实验组新生毛囊形成正确结构。此外，混合物注射诱导出规则的、多层的复层表皮以及含有毛囊样结构的表皮。我们的数据表明，注射表皮干细胞和真皮乳头细胞的混合物可以诱导毛囊再生和有序的表皮形成。这项研究强调，种子细胞和种子细胞生态位之间相互作用的重新排列对于组织工程构建的成功至关重要。