The present study evaluated revascularization time of fresh and cryopreserved cat ovarian tissue after transplantation to subcutaneous tissue. Ovaries of five cats were used and eight pieces of ovarian tissue were taken from each pair of ovaries. Immediately after removal, three pieces were transplanted and one fixed for fresh control. The remaining four pieces were cryopreserved and, after thawing, one was fixed for cryopreservation control and three were transplanted. Grafts were recovered on days 2 (D2), 4 (D4) and 6 (D6) post-transplantation. Blood vessels were identified by immunohistochemistry and doppler ultrasound. Immunohistochemistry showed that the percentages of total tissue area occupied by blood vessels were similar (P > 0.05) in fresh and cryopreserved tissues. In both cases, blood vessel area was significantly higher (P < 0.05) on D4 and D6 compared to D0. Ultrasound analysis showed vascularization improvement on the periphery of grafts from D2 to D4 and from D4 to D6, both in fresh and cryopreserved tissue samples. Nonetheless, there was a significant decrease (P < 0.05) in the percentage of morphologically normal follicles (MNF) after transplantation compared to non-transplanted tissue (D0), both for fresh and cryopreserved samples. Moreover, the number of follicles found in samples was considerably smaller after grafting. In conclusion, revascularization of ovarian tissue autotransplanted to subcutaneous tissue in domestic cats occurs within 4 days after transplantation, both for fresh and cryopreserved tissue. However, large follicular loss has been observed in the first days post-transplantation, especially in cryopreserved tissues.

中文翻译：

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家猫新鲜和冻存卵巢组织移植后血运重建时间和初始损伤的研究。

本研究评估了新鲜和冷冻保存的猫卵巢组织移植到皮下组织后的血运重建时间。使用五只猫的卵巢，并从每对卵巢中取出八块卵巢组织。取出后立即移植三块，并固定一块作为新鲜对照。其余四块被冷冻保存，解冻后，固定一块用于冷冻保存对照，三块被移植。移植物在移植后第 2 天 (D2)、第 4 天 (D4) 和第 6 天 (D6) 恢复。通过免疫组织化学和多普勒超声鉴定血管。 免疫组化结果显示，新鲜组织和冻存组织中血管所占组织总面积的百分比相似（P >0.05）。 在这两种情况下，与 D0 相比，D4 和 D6 的血管面积显着更高 ( P < 0.05)。超声分析显示，新鲜和冷冻保存的组织样本中，从 D2 到 D4 以及从 D4 到 D6 的移植物周围血管化均有所改善。 尽管如此，无论是新鲜样本还是冷冻样本，与非移植组织 (D0) 相比，移植后形态正常卵泡 (MNF) 的百分比均显着下降( P < 0.05)。此外，移植后样本中发现的毛囊数量明显减少。总之，家猫卵巢组织自体移植到皮下组织的血运重建发生在移植后 4 天内，无论是新鲜组织还是冷冻保存组织。然而，在移植后的第一天就观察到大量的卵泡损失，特别是在冷冻保存的组织中。