Abstract

The physicochemical properties of pressurized hot-water extract (PHE) from abalone muscle and the antioxidant activities of its digestive products were investigated in this study. The PHE contained protein of 49.58% and carbohydrate of 41.95%. After ethanol graded precipitation, most of carbohydrate and protein in PHE were successively remained in 40% ethanol precipitate (EP40) and 80% ethanol precipitate (EP80), respectively. High proportions of Glu and Gly were found in the PHE, EP40 and EP80, but the proportion of Ala in ethanol soluble extract (ESE) reached up to 46.00%. Both PHE and EP40 were rich in glucose, while galactose and glucose were main monosaccharides in the EP80 and ESE. Based on the results of SDS-PAGE and HPLC, high molecular weight components from PHE were precipitated in the EP40, but oligopeptides and free amino acids were fractionated in EP80 and ESE. Among the PHE and the digestive products, the highest antioxidant ability was found to be EP80 hydrolysate, the IC₅₀ values of which for scavenging activity on hydroxyl radical, DMPD radical and ABTS radical were 1.05 mg/mL, 1.40 mg/mL and 0.56 mg/mL, respectively. It is concluded that carbohydrate of abalone muscle was dissolved more easily into hot water than protein, and protein hydrolysate of PHE might play an important role in antioxidant activity of gastrointestinal digestion.

中文翻译：

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鲍鱼肌肉加压热水提取物特性及体外模拟消化过程中的抗氧化能力

摘要

本研究研究了鲍鱼肌肉加压热水提取物（PHE）的理化性质及其消化产物的抗氧化活性。PHE含有49.58%的蛋白质和41.95%的碳水化合物。乙醇分级沉淀后，PHE中的大部分碳水化合物和蛋白质依次保留在40%乙醇沉淀（EP40）和80%乙醇沉淀（EP80）中。在PHE、EP40和EP80中发现了高比例的Glu和Gly，但乙醇可溶提取物(ESE)中Ala的比例高达46.00%。PHE和EP40都富含葡萄糖，而半乳糖和葡萄糖是EP80和ESE中的主要单糖。根据 SDS-PAGE 和 HPLC 的结果，来自 PHE 的高分子量组分在 EP40 中沉淀，但寡肽和游离氨基酸在 EP80 和 ESE 中被分离。在 PHE 和消化产物中，发现抗氧化能力最高的是 EP80 水解产物，IC_其中50个对羟基自由基、DMPD自由基和ABTS自由基的清除活性值分别为1.05mg/mL、1.40mg/mL和0.56mg/mL。结论鲍鱼肌肉中的碳水化合物比蛋白质更容易溶解在热水中，PHE的蛋白质水解物可能在胃肠消化的抗氧化活性中起重要作用。