Abstract For this study, we used leaves of fossilized Metasequoia nathorstii from the Middle Eocene sediments of the Axel Heiberg Island of the Canadian Arctic Archipelago, and the leaves of modern M. glyptostroboides collected from the garden of the Komarov Botanical Institute RAS. Feulgen staining of the Metasequoia fossil leaves revealed the preservation of Feulgen-positive, heterogeneously stained nuclear structures in the cells, similar to those in the cells of the modern plant. The nuclei were brown-red-stained, and the chromocenters were stained more intensely, suggesting that the general morphology of the cell nucleus was preserved and the aldehyde groups of the deoxyribose that were available to react with the Feulgen's basic fuchsin were preserved in the nuclear chromatin of the fossil plants. The additional staining of the preparations with the alcian blue and Ehrlich's hematoxylin displays the cell walls and the adjacent intracellular material that is colored from light to dark blue. Staining the preparations with dA + dT-specific fluorochrome DAPI induced a weak fluorescence of the fossil cell nuclei, indicating the presence of some dsDNA molecules in the Metasequoia fossil leaves.

中文翻译：

---

使用 DNA 特异性染色剂作为加拿大北极中始新世水杉叶细胞核和核外物质的指示剂

摘要 在这项研究中，我们使用了来自加拿大北极群岛 Axel Heiberg 岛中始新世沉积物的 Metasequoia nathorstii 的叶子，以及从 Komarov Botanical Institute RAS 花园收集的现代 M. glyptostroboides 的叶子。水杉化石叶子的 Feulgen 染色揭示了细胞中 Feulgen 阳性、异质染色的核结构的保存，类似于现代植物细胞中的结构。细胞核呈棕红色，染色中心染色更强烈，表明细胞核的一般形态得以保留，可与费尔根碱性品红反应的脱氧核糖醛基保留在细胞核中。化石植物的染色质。用阿尔新蓝和埃利希苏木精对制剂进行额外染色，显示细胞壁和相邻的细胞内物质，其颜色从浅蓝色到深蓝色。用 dA + dT 特异性荧光染料 DAPI 对制剂进行染色会诱导化石细胞核的微弱荧光，表明水杉化石叶子中存在一些 dsDNA 分子。