Isoflavones, such as genistein, have been proposed to have beneficial effects on health, including preventive or therapeutic actions in carcinogenesis. Their structural similarity to oestrogens allows them to bind at the cellular level with oestrogen receptors. Therefore, this study attempted to determine the antitumoural effects of genistein administered in a canine inflammatory mammary cancer xenograft model, in terms of tumour proliferation, appearance of metastases and steroid hormone regulation. Using histology and immunohistochemical analyses as well as the EIA technique for hormonal determinations, the antitumoural effects of genistein on an inflammatory mammary cancer xenograft model were assessed for 3 weeks. Mice treated with genistein showed higher Ki-67 levels than the control group. There were significantly more distant metastases in the genistein-treated xenografts versus the control group. Intratumoural and serum progesterone, androstenedione and oestrogen levels in treated mice were elevated, whereas intratumoural testosterone levels were decreased compared to the control group. These results revealed that genistein ingestion promotes tumour proliferation and elevates metastatic rates by increasing intratumoural and circulating oestrogen levels in a mammary cancer xenograft model.

中文翻译：

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金雀异黄素治疗犬炎症性乳腺癌异种移植小鼠后类固醇激素谱和肿瘤进展的变化。

异黄酮（如染料木黄酮）已被建议对健康具有有益作用，包括在致癌作用中的预防或治疗作用。它们与雌激素的结构相似性使它们可以在细胞水平上与雌激素受体结合。因此，本研究试图从肿瘤增殖，转移灶的出现和类固醇激素调节方面确定染料木黄酮在犬类炎症性乳腺癌异种移植模型中的抗肿瘤作用。使用组织学和免疫组织化学分析以及EIA技术进行激素测定，在3周的时间内评估了染料木黄酮对炎症性乳腺癌异种移植模型的抗肿瘤作用。用染料木黄酮处理的小鼠的Ki-67水平高于对照组。用染料木黄酮处理的异种移植物与对照组相比，远处转移更为明显。与对照组相比，治疗小鼠的肿瘤内和血清孕酮，雄烯二酮和雌激素水平升高，而肿瘤内睾丸激素水平降低。这些结果表明，在乳腺癌异种移植模型中，染料木黄酮的摄取通过增加肿瘤内和循环中的雌激素水平来促进肿瘤增殖并提高转移率。