Spent silkworm pupa is a valuable source of alternative protein in poultry production. Usually the spent silkworm pupae are wasted and may cause environmental pollution and off smell. This feeding experiment was performed to determine the gradual increase of silkworm substitution of soybean on the egg production performance, serum biochemistry and intestinal histological features of White Leghorns laying hens. For this purpose, a total of 250 White Leghorn laying hens were allocated to five experimental treatments. Rations were prepared in which soybean meal was replaced with 0, 25, 50, 75 and 100% substitution with silkworm meal. The outcomes of the study indicated that performance was not significantly (P < .05) different among the experimental groups. Aspartate aminotransferase (AST) and alanine aminotrasferase (ALT), uric acid and creatinin concentration did not significantly (P > .05) alter among the groups during the experimental period. Similarly, height and thickness of intestinal villi, surface of intestinal villi, number of goblet cells did not change between the control and experimental groups. It was concluded that substitution of silkworm meal with soybean meal had no hostile effect on production performance, intestinal histomorphology and blood biochemical functions of liver and kidney in White Leghorn hens.

废蚕蛹是家禽生产中替代蛋白质的宝贵来源。通常，用过的蚕蛹被浪费掉，可能会造成环境污染和异味。本饲喂试验旨在确定蚕替代大豆的逐渐增加对白来航蛋鸡产蛋性能、血清生化和肠道组织学特征的影响。为此，总共 250 只白来航蛋鸡被分配到五个实验处理组。制备了用蚕粉0％、25％、50％、75％和100％替代豆粕的日粮。研究结果表明，实验组之间的表现没有显着差异 ( P < .05)。实验期间，各组间天冬氨酸转氨酶（AST）和丙氨酸转氨酶（ALT）、尿酸和肌酐浓度没有显着变化（ P > .05）。同样，对照组和实验组的肠绒毛高度和厚度、肠绒毛表面、杯状细胞数量也没有变化。结论：蚕粉替代豆粕对白来亨鸡生产性能、肠道组织形态及肝肾血液生化功能无不良影响。