Abstract In this paper, the antibiofilm activity of microelectrode arrays (MEAs) related to their physicochemical properties was studied. Our objective was to determine whether any of the MEAs in contact with the biofilms elicit a considerable antibiofilm activity. Our hypothesis was that the antibiofilm activity of the MEAs depended on the materials used for the preparation of their tips. The antibiofilm activity of the samples was determined by measuring the ratio of the killed to alive bacteria incubated with microelectrodes. To our knowledge, the antibiofilm activity of MEAs related to their physicochemical properties has not been reported, yet. The use of different tips made of carbon nanotube (CNT) or platinum (Pt) for the samples allowed in situ monitoring of their antibiofilm activity. The complementary data of FTIR and SEM analysis of the samples before and after incubation with the biofilm revealed the attachment of proteins from biofilm on the sample tips. The confocal microscopy showed that the ratio of dead to alive cells incubated with the MEAs coated with parylene-C, the samples with Pt tips and the ones with CNT tips was 15.2% ± 9.8%, 40.4% ± 12.3% and 25.8% ± 8.2%, respectively. This study presented the evidence of the effect of type of sample tips on the anti-biofilm effect of MEAs. These results suggest that MEAs with Pt tips, killing more bacteria than the ones with CNT tips or without coated tips, could be used for the preparation of sterile surfaces of these implants.

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微电极阵列抗生物膜活性和理化性质的比较研究

摘要 本文研究了微电极阵列 (MEA) 的抗生物膜活性与其理化性质相关。我们的目标是确定与生物膜接触的任何 MEA 是否会引起相当大的抗生物膜活性。我们的假设是 MEA 的抗生物膜活性取决于用于制备其尖端的材料。样品的抗生物膜活性通过测量与微电极一起培养的被杀死的细菌与活的细菌的比率来确定。据我们所知，尚未报道与其理化特性相关的 MEA 的抗生物膜活性。使用由碳纳米管 (CNT) 或铂 (Pt) 制成的不同尖端用于样品允许原位监测其抗生物膜活性。与生物膜孵育前后样品的 FTIR 和 SEM 分析的补充数据揭示了来自生物膜的蛋白质附着在样品尖端上。共聚焦显微镜显示，用涂有聚对二甲苯-C 的 MEA、带有 Pt 尖端的样品和带有 CNT 尖端的样品孵育的死细胞与活细胞的比例分别为 15.2% ± 9.8%、40.4% ± 12.3% 和 25.8% ± 8.2 ％， 分别。本研究提供了样品尖端类型对 MEA 抗生物膜效应影响的证据。这些结果表明，具有 Pt 尖端的 MEA 比具有 CNT 尖端或没有涂层尖端的 MEA 杀死更多的细菌，可用于制备这些植入物的无菌表面。共聚焦显微镜显示，用涂有聚对二甲苯-C 的 MEA、带有 Pt 尖端的样品和带有 CNT 尖端的样品孵育的死细胞与活细胞的比例分别为 15.2% ± 9.8%、40.4% ± 12.3% 和 25.8% ± 8.2 ％， 分别。本研究提供了样品尖端类型对 MEA 抗生物膜作用影响的证据。这些结果表明，具有 Pt 尖端的 MEA 比具有 CNT 尖端或没有涂层尖端的 MEA 杀死更多的细菌，可用于制备这些植入物的无菌表面。共聚焦显微镜显示，用涂有聚对二甲苯-C 的 MEA、带有 Pt 尖端的样品和带有 CNT 尖端的样品孵育的死细胞与活细胞的比例分别为 15.2% ± 9.8%、40.4% ± 12.3% 和 25.8% ± 8.2 ％， 分别。本研究提供了样品尖端类型对 MEA 抗生物膜效应影响的证据。这些结果表明，具有 Pt 尖端的 MEA 比具有 CNT 尖端或没有涂层尖端的 MEA 杀死更多的细菌，可用于制备这些植入物的无菌表面。