The bactericidal properties of bacteriophages have been used almost since the moment of the discovery of bacterial viruses. In the light of the rapidly growing number of antibiotic-resistant bacteria, phage therapy is considered one of the most promising alternatives to classical treatment. Phage amplification is one of the most common procedures of working with phages, and high-titer preparations are beneficial at the experimental stage of studies as well as in practice. The objective of this study was to compare five commonly applied methods of phage amplification: (i) pooled plaques method, (ii) the plate wash method, (iii) the agar culture method, (iv) the two-stage culture method, and (v) in liquid culture. All methods were tested for fifteen different phages. The results described herein indicate that there is no optimal, universal method for phage amplification, and the most effective method has to be established individually for each phage.

几乎从细菌病毒被发现的那一刻起，噬菌体的杀菌特性就被人们所利用。鉴于抗生素耐药细菌数量迅速增加，噬菌体疗法被认为是经典治疗最有前途的替代方案之一。噬菌体扩增是使用噬菌体的最常见程序之一，高滴度制剂在研究的实验阶段以及实践中都是有益的。本研究的目的是比较五种常用的噬菌体扩增方法：(i) 混合噬菌斑法、(ii) 洗板法、(iii) 琼脂培养法、(iv) 两阶段培养法和(v)液体培养。所有方法均针对十五种不同的噬菌体进行了测试。本文描述的结果表明，噬菌体扩增没有最佳的、通用的方法，并且必须为每个噬菌体单独建立最有效的方法。