Wide‐scale evaluation of Origanum munzurense Kit Tan & Sorger using different extraction techniques: Antioxidant capacity, chemical compounds, trace element content, total phenolic content, antibacterial activity and genotoxic effect,Flavour and Fragrance Journal

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Wide‐scale evaluation of Origanum munzurense Kit Tan & Sorger using different extraction techniques: Antioxidant capacity, chemical compounds, trace element content, total phenolic content, antibacterial activity and genotoxic effect
Flavour and Fragrance Journal ( IF 2.1 ) Pub Date : 2020-04-16 , DOI: 10.1002/ffj.3574
Erdal Yabalak ₁ , Zuhal Emire ₁ , Ali Osman Adıgüzel ₂ , Serpil Könen Adıgüzel ₃ , A. Murat Gizir ₁

Affiliation

This aim of this work was to investigate the various properties of Origanum munzurense Kit Tan & Sorger that are previously unknown. In this context, chemical compound, antioxidant activity, total polyphenol content, mineral and trace element composition, antibacterial activity and genotoxicity effects of its extracts and essential oil were widely analysed. The extracts and essential oil of Origanum munzurense were obtained by three different extraction methods. All contents of extracts and essential oil were analysed by GC‐MS and evaluated according to mass spectral libraries of GC‐MS. The identified compounds were evaluated according to literature to put forward their benefits on human health. The phenolic content analysis of Origanum munzurense was performed using the Folin‐Ciocalteu method. 178.59, 184.20, 231.17, 319.28 and 1051.94 mg GAE/100 g dry weight were determined for methanol, water, acetonitrile, ethanol and subcritical water extracts, respectively. DPPH and CUPRAC methods were used to determine antioxidant activity. The highest half‐maximal inhibitory concentration and Trolox equivalent antioxidant capacity values were found as 0.110 mg mL⁻¹ and 2.197 using water and tetrahydrofuran extracts, respectively. A total of 21 mineral and trace elements were detected in the ICP‐MS analysis of O munzurense using the wet digestion procedure. The effects of the extracts and essential oil on Klebsiella pneumoniae, Escherichia coli, Pseudomonas aeruginosa and S aureus were examined using disc diffusion and microdilution methods. Essential oil exhibited the highest antibacterial activity. The essential oil was found to cause less DNA damage using the comet assay in Hep G2 cells compared with tetrahydrofuran extract.

中文翻译：

使用不同的提取技术对牛至花药材Tan＆Sorger进行大规模评估：抗氧化能力，化学成分，微量元素含量，总酚含量，抗菌活性和遗传毒性作用

这项工作的目的是调查以前未知的牛至植物牛油菌Tan和Sorger的各种特性。在这种情况下，广泛分析了其提取物和精油的化合物，抗氧化剂活性，总多酚含量，矿物质和微量元素组成，抗菌活性和遗传毒性作用。通过三种不同的提取方法获得了牛至的提取物和香精油。提取物和精油的所有含量均通过GC-MS分析，并根据GC-MS的质谱库进行评估。根据文献对鉴定出的化合物进行评估，以提出其对人体健康的益处。牛至的酚含量分析使用Folin-Ciocalteu方法进行。对于甲醇，水，乙腈，乙醇和亚临界水提取物，分别测定了178.59、184.20、231.17、319.28和1051.94 mg GAE / 100 g干重。使用DPPH和CUPRAC方法确定抗氧化活性。使用水和四氢呋喃提取物得到的最高最大半数抑制浓度和Trolox当量抗氧化剂容量值分别为0.110 mg mL ^-1和2.197。ICP-MS湿消解法在芒草的ICP-MS分析中总共检测到21种矿物质和微量元素。提取物和精油对肺炎克雷伯菌，大肠杆菌，铜绿假单胞菌的影响。使用圆盘扩散法和微稀释法检查金黄色葡萄球菌和金黄色葡萄球菌。香精油表现出最高的抗菌活性。与四氢呋喃提取物相比，使用彗星测定法在Hep G2细胞中发现精油对DNA的损伤较小。

更新日期：2020-04-16

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