Quantitative three-dimensional nondestructive imaging of whole anaerobic ammonium-oxidizing bacteria.,Journal of Synchrotron Radiation

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Quantitative three-dimensional nondestructive imaging of whole anaerobic ammonium-oxidizing bacteria.
Journal of Synchrotron Radiation ( IF 2.4 ) Pub Date : 2020-04-17 , DOI: 10.1107/s1600577520002349
Meng Wen Peng ₁ , Yong Guan ₂ , Jian Hong Liu ₂ , Liang Chen ₂ , Han Wang ₁ , Zheng Zhe Xie ₃ , Hai Yan Li ₁ , You Peng Chen ₁ , Peng Liu ₄ , Peng Yan ₁ , Jin Song Guo ₁ , Gang Liu ₂ , Yu Shen ₅ , Fang Fang ₁

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Anaerobic ammonium‐oxidizing (anammox) bacteria play a key role in the global nitrogen cycle and in nitrogenous wastewater treatment. The anammox bacteria ultrastructure is unique and distinctly different from that of other prokaryotic cells. The morphological structure of an organism is related to its function; however, research on the ultrastructure of intact anammox bacteria is lacking. In this study, in situ three‐dimensional nondestructive ultrastructure imaging of a whole anammox cell was performed using synchrotron soft X‐ray tomography (SXT) and the total variation‐based simultaneous algebraic reconstruction technique (TV‐SART). Statistical and quantitative analyses of the intact anammox bacteria were performed. High soft X‐ray absorption composition inside anammoxosome was detected and verified to be relevant to iron‐binding protein. On this basis, the shape adaptation of the anammox bacteria response to iron was explored.

中文翻译：

整个厌氧氨氧化细菌的定量三维无损成像。

厌氧氨氧化（anammox）细菌在全球氮循环和含氮废水处理中发挥着关键作用。厌氧氨氧化细菌的超微结构是独特的，与其他原核细胞明显不同。生物体的形态结构与其功能有关；然而，对完整厌氧氨氧化菌超微结构的研究还很缺乏。在这项研究中，使用同步加速器软X射线断层扫描（SXT）和基于全变分的同时代数重建技术（TV-SART）对整个厌氧氨氧化细胞进行原位三维无损超微结构成像。对完整的厌氧氨氧化细菌进行了统计和定量分析。检测到厌氧氨氧化体内的高软X射线吸收成分，并验证其与铁结合蛋白相关。在此基础上，探索了厌氧氨氧化菌对铁反应的形状适应性。

更新日期：2020-04-17

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