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Characterization of partially purified alkaloids from Cucurbita maxima seed and evaluation of their antioxidant activity in human erythrocytes and leukocytes.
Journal of Food Biochemistry ( IF 4 ) Pub Date : 2020-04-17 , DOI: 10.1111/jfbc.13225
Palanisamy Vasudhevan 1 , Manoharan Manikandan 2 , Palaniswamy Thangavel 1 , Sasikumar Vadivukkarasi 2
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Cucurbita maxima, is a therapeutic plant spread all over the world. The seed of C. maxima constitutes a large amount of alkaloids, phenolic compounds, Vitamin E, and other secondary metabolites. The experiment was performed in four setups (Control, Only H2O2, H2O2 + 25 mg of alkaloid, H2O2 + 50 mg of alkaloids). The enzymatic and nonenzymatic antioxidants and lipid peroxidation (LPO) were determined to analyze the antioxidant effect of partially purified alkaloids. The results showed that partially purified alkaloids from C. maxima seed reacts effectively on the erythrocytes and leukocytes antioxidant enzyme system when compared to the hydrogen peroxide group. The present results indicate that the alkaloids present in this plant can be used as a natural antioxidant for the pharmacological purposes.

中文翻译:

最大南瓜属种子部分纯化的生物碱的表征及其在人红细胞和白细胞中抗氧化活性的评估。

西葫芦,是遍布世界各地的治疗植物。C. maxima的种子构成了大量的生物碱,酚类化合物,维生素E和其他次生代谢产物。实验以四种设置进行(对照,仅H 2 O 2,H 2 O 2  + 25 mg生物碱,H 2 O 2  + 50 mg生物碱)。确定了酶促和非酶促抗氧化剂和脂质过氧化(LPO),以分析部分纯化的生物碱的抗氧化作用。结果表明,部分纯化的最大生物碱与过氧化氢基团相比,种子对红细胞和白细胞的抗氧化酶系统有效反应。目前的结果表明存在于该植物中的生物碱可以作为天然抗氧化剂用于药理学目的。
更新日期:2020-04-17
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