In this study, we strived to investigate the effect of miR-205-5p on JAK/STAT signaling way induced by P. gingivalis in periodontitis. Microarray analysis was conducted to find differentially expressed miRNAs in periodontitis patients. The miRNAs related to JAK/STAT signaling way were selected via DIANA TOOLS, and the targeted mRNAs of miRNAs were predicted by TargetScan. The expression of miRNAs and mRNAs, differentially expressed in periodontitis and related to JAK/STAT signaling, was detected by qRT-PCR or western blot. The relationship between miRNAs and mRNAs was confirmed by a dual luciferase assay. MiR-205-5p was downregulated and IL6ST was upregulated in periodontitis patients' clinical samples. MiR-205-5p had target binding sites of IL6ST 3′ untranslated region. QRT-PCR and western blot analysis demonstrated poor expression of miR-205-5p, while IL6ST, pJAK2, p-STAT3 were extremely upregulated in gingival epithelial cells (GECs) with P. gingivalis induction. IL6ST expression in periodontitis tissue was also increased. P. gingivalis could inhibit miR-205-5p expression to activate JAK/STAT signaling in GECs and promote the occurrence and development of periodontitis.

中文翻译：

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牙龈卟啉单胞菌对MicroRNA-205-5p表达的抑制作用可调节牙龈上皮细胞中的促炎细胞因子。

在这项研究中，我们努力研究miR-205-5p对牙龈卟啉单胞菌诱导的JAK / STAT信号转导途径的影响。在牙周炎。进行微阵列分析以发现牙周炎患者中差异表达的miRNA。通过DIANA TOOLS选择与JAK / STAT信号转导方式相关的miRNA，并通过TargetScan预测miRNA的靶向mRNA。通过qRT-PCR或Western印迹检测在牙周炎中差异表达并与JAK / STAT信号相关的miRNA和mRNA的表达。通过双重荧光素酶测定法证实了miRNA和mRNA之间的关系。在牙周炎患者的临床样本中，MiR-205-5p被下调，IL6ST被上调。MiR-205-5p具有IL6ST 3'非翻译区的靶结合位点。QRT-PCR和Western印迹分析表明miR-205-5p的表达较差，而IL6ST，pJAK2，p-STAT3在牙龈上皮细胞（GEC）中的表达却极高。牙龈卟啉单胞菌的诱导。牙周炎组织中的IL6ST表达也增加。牙龈卟啉单胞菌可抑制miR-205-5p表达，以激活GEC中的JAK / STAT信号传导，并促进牙周炎的发生和发展。