The peritoneum lines the abdominal cavity, covering and supporting the abdominal organs. The layer of mesothelial cells provides a functional barrier and allows vectorial transport between serous fluid of the abdominal cavity and tissue fluid. Based on the anatomical localization, three peritoneal regions can be distinguished: parietal, visceral and diaphragmatic. However, a comparative analysis of their barrier and transport properties has not yet been carried out. Electrophysiological parameters of three different regions of the rat peritoneum were investigated here using the Ussing chamber. The parietal peritoneum revealed the highest transmesothelial potential (2.2 ± 0.3 mV), short circuit current (19.8 ± 1.7 мA/cm 2 ), and transmesothelial resistance (94.9 ± 3.5 Ohm cm 2 ) compared to other peritoneal regions. The addition of ouabain (1 mM) from the apical and basolateral sides of the parietal and visceral peritoneum resulted in an increase in the transmesothelial resistance. In addition, a histological analysis was performed. Tissue preparations of the parietal peritoneum comprised a layer of mesothelial cells and adjacent striated muscle fibers with small interlayers of loose connective tissue. Tissue specimens of the diaphragmatic and visceral peritonea included two layers of mesothelial cells. In the diaphragmatic peritoneum, they were separated by muscle fibers and large areas of loose connective tissue, while in the visceral peritoneum by adipose and connective tissues. In conclusion, the parietal and visceral regions of the rat peritoneum contribute differentially to transmesothelial transport. The parietal peritoneum exhibits pronounced barrier and transport properties and can be considered as a promising model for studying the molecular interaction between Na/K-ATPase and tight junction proteins, claudins.

中文翻译：

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大鼠腹膜不同区域的电生理参数

腹膜位于腹腔内，覆盖并支撑腹部器官。间皮细胞层提供了一个功能屏障，并允许腹腔浆液和组织液之间的矢量传输。根据解剖定位，可以区分三个腹膜区域：顶叶、内脏和膈肌。然而，尚未对它们的阻隔和传输特性进行比较分析。此处使用 Ussing 室研究了大鼠腹膜三个不同区域的电生理参数。与其他腹膜区域相比，壁层腹膜显示出最高的跨间皮电位 (2.2 ± 0.3 mV)、短路电流 (19.8 ± 1.7 мA/cm 2 ) 和跨间皮电阻 (94.9 ± 3.5 Ohm cm 2 )。从顶叶和内脏腹膜的顶端和基外侧添加哇巴因 (1 mM) 导致跨间皮电阻增加。此外，还进行了组织学分析。壁层腹膜的组织制剂包括一层间皮细胞和相邻的横纹肌纤维，中间有小的疏松结缔组织。膈肌和内脏腹膜的组织标本包括两层间皮细胞。在膈腹膜中，它们被肌肉纤维和大面积疏松结缔组织隔开，而在内脏腹膜中被脂肪和结缔组织隔开。总之，大鼠腹膜的顶叶和内脏区域对跨间皮转运的贡献不同。