The rice blast fungus Magnaporthe oryzae differentiates a specialized infection structure called an appressorium, which is used to break into plant cells by directed application of enormous turgor force. Appressorium-mediated plant infection requires timely assembly of a higher-order septin ring structure at the base of the appressorium, which is needed to spatially orchestrate appressorium repolarization. Here we use quantitative 4D widefield fluorescence imaging to gain new insight into the spatiotemporal dynamics of septin ring formation, and septin-mediated actin re-organization, during appressorium morphogenesis by M. oryzae. We anticipate that the new knowledge will provide a quantitative framework for dissecting the molecular mechanisms of higher-order septin ring assembly in this devastating plant pathogenic fungus.

中文翻译：

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稻瘟病真菌在附着胞形态发生过程中动态组装高级 septin 结构。

稻瘟病真菌 Magnaporthe oryzae 区分出一种称为附着物的特殊感染结构，用于通过直接施加巨大的膨胀力来侵入植物细胞。附着胞介导的植物感染需要在附着胞基部及时组装高阶 septin 环结构，这是空间协调附着胞复极化所必需的。在这里，我们使用定量 4D 宽场荧光成像来获得对 septin 环形成和 septin 介导的肌动蛋白重组的时空动力学的新见解，在 M. oryzae 的附着物形态发生过程中。我们预计新知识将为剖析这种破坏性植物病原真菌中高级 septin 环组装的分子机制提供定量框架。