Molecular Metabolism ( IF 7.0 ) Pub Date : 2020-04-17 , DOI: 10.1016/j.molmet.2020.100998 Jonas R Knudsen 1 , Dorte E Steenberg 2 , Janne R Hingst 2 , Lorna R Hodgson 3 , Carlos Henriquez-Olguin 2 , Zhencheng Li 2 , Bente Kiens 2 , Erik A Richter 2 , Jørgen F P Wojtaszewski 2 , Paul Verkade 3 , Thomas E Jensen 2
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Objective
Exercise is a cornerstone in the management of skeletal muscle insulin-resistance. A well-established benefit of a single bout of exercise is increased insulin sensitivity for hours post-exercise in the previously exercised musculature. Although rodent studies suggest that the insulin-sensitization phenomenon involves enhanced insulin-stimulated GLUT4 cell surface translocation and might involve intramuscular redistribution of GLUT4, the conservation to humans is unknown.
Methods
Healthy young males underwent an insulin-sensitizing one-legged kicking exercise bout for 1 h followed by fatigue bouts to exhaustion. Muscle biopsies were obtained 4 h post-exercise before and after a 2-hour hyperinsulinemic-euglycemic clamp.
Results
A detailed microscopy-based analysis of GLUT4 distribution within seven different myocellular compartments revealed that prior exercise increased GLUT4 localization in insulin-responsive storage vesicles and T-tubuli. Furthermore, insulin-stimulated GLUT4 localization was augmented at the sarcolemma and in the endosomal compartments.
Conclusions
An intracellular redistribution of GLUT4 post-exercise is proposed as a molecular mechanism contributing to the insulin-sensitizing effect of prior exercise in human skeletal muscle.
中文翻译：
事先在人体中运动会重新分布肌内GLUT4，并增强胰岛素刺激的肌膜和内体GLUT4的转运。
目的
运动是管理骨骼肌胰岛素抵抗的基石。一次运动的公认好处是，在先前运动过的肌肉组织中运动后数小时，胰岛素敏感性增加。尽管啮齿动物的研究表明，胰岛素增敏现象涉及胰岛素刺激的GLUT4细胞表面转运的增强，并且可能涉及GLUT4的肌内重新分布，但对人类的保护尚不清楚。
方法
健康的年轻男性接受胰岛素敏感的单腿踢腿运动1小时，然后疲劳疲惫。运动后4小时，在2小时高胰岛素-正常血糖钳夹前后，进行肌肉活检。
结果
基于显微镜的详细分析，分析了七个不同肌细胞区室中GLUT4的分布情况，表明先前的锻炼增加了GLUT4在胰岛素反应性囊泡和T小管中的定位。此外，胰岛素刺激的GLUT4定位在肌膜和内体区室中增加。
结论
运动后GLUT4的细胞内重新分布被提议为有助于人类骨骼肌先前运动的胰岛素增敏作用的分子机制。
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