Thioproline Serves as an Efficient Antioxidant Protecting Human Cells from Oxidative Stress and Improves Cell Viability.,Chemical Research in Toxicology

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Thioproline Serves as an Efficient Antioxidant Protecting Human Cells from Oxidative Stress and Improves Cell Viability.
Chemical Research in Toxicology ( IF 3.7 ) Pub Date : 2020-04-17 , DOI: 10.1021/acs.chemrestox.0c00055
Yat-Hing Ham ₁ , K K Jason Chan ₁ , Wan Chan ₁

Affiliation

Oxidative stress is associated with the pathophysiology of many degenerative human diseases, including Alzheimer’s disease, atherosclerosis, Parkinson’s disease, and cancers. We discovered in our previous study that thioproline (SPro), a proline analogue, is generated in oxidant-exposed cells. With the prior observation that SPro served as an efficient nitrile trapping agent, we tested in this study the hypothesis that this oxidative stress generated cysteine-formaldehyde adduct, SPro, may serve as an antioxidant protecting cells from oxidative stress. Interestingly, results showed that HeLa cells cultured in SPro-supplemented culture media are more tolerant of oxidative stress, indicated by a dosage-dependent increase in cell viability. Investigation of the molecular mechanism of the observed increase in cell tolerance to oxidative stress revealed SPro acting as an effective antioxidant by sacrificial oxidation. Results also showed that SPro had been incorporated into cellular proteins and induced changes in protein expression profiles of treated cells. Despite being yet to determine the participation of individual factors to the observed increase of cell tolerance to oxidative stress, this study sheds light on the potential use of SPro as a dietary supplement for protecting humans from oxidative stress-associated degenerative human diseases.

中文翻译：

硫代脯氨酸作为一种有效的抗氧化剂，可保护人体细胞免受氧化应激并提高细胞活力。

氧化应激与许多退化性人类疾病的病理生理相关，包括阿尔茨海默氏病，动脉粥样硬化，帕金森氏病和癌症。我们在之前的研究中发现，脯氨酸类似物硫代脯氨酸（SPro）在暴露于氧化剂的细胞中生成。通过先前观察到SPro可以作为一种有效的腈捕获剂，我们在本研究中测试了以下假设：该氧化应激产生的半胱氨酸-甲醛加合物SPro可以充当抗氧化剂，保护细胞免受氧化应激的影响。有趣的是，结果表明，在补充了SPro的培养基中培养的HeLa细胞对氧化应激的耐受性更高，这表现为细胞活力的剂量依赖性增加。对观察到的细胞对氧化应激的耐受性增加的分子机制的研究表明，SPro通过牺牲氧化可作为有效的抗氧化剂。结果还表明，SPro已被掺入细胞蛋白质中，并诱导了处理过的细胞蛋白质表达谱的变化。尽管尚未确定单个因素是否参与了观察到的细胞对氧化应激耐受性的增加，但这项研究为SPro作为膳食补充剂在保护人类免受与氧化应激相关的退化性人类疾病的饮食中的潜在用途提供了启示。

更新日期：2020-04-17

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