Transporter Engineering for Microbial Manufacturing.,Biotechnology Journal

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Transporter Engineering for Microbial Manufacturing.
Biotechnology Journal ( IF 3.2 ) Pub Date : 2020-04-16 , DOI: 10.1002/biot.201900494
Ying Zhu ₁ , Chen Zhou ₁ , Ying Wang ₁ , Chun Li ₁

Affiliation

Microbes play an important role in biotransformation and biosynthesis of biofuels, natural products, and polymers. Therefore, microbial manufacturing has been widely used in medicine, industry, and agriculture. However, common strategies including enzyme engineering, pathway optimization, and host engineering are generally inadequate to obtain an efficient microbial production system. Transporter engineering provides an alternative strategy to promote the transmembrane transfer of substrates, intermediates, and final products in microbial cells and thus enhances production by alleviating feedback inhibition and cytotoxicity caused by final products. According to the current studies in transport engineering, native transporters usually have low expression and poor transportation ability, resulting in inefficient transport processes and microbial production. In this review, current approaches for transporter mining, characterization, and verification are comprehensively summarized. Practical approaches to enhance the transport system in engineered cells, such as balancing transporter overexpression and cell growth, and evolution of native transporters are discussed. Furthermore, the applications of transporter engineering in microbial manufacturing, including enhancement of substrate utilization, concentration of metabolic flux to the target pathway, and acceleration of efflux and recovery of products, demonstrate its outstanding advantages and promising prospects.

中文翻译：

微生物制造转运技术。

微生物在生物燃料，天然产物和聚合物的生物转化和生物合成中起着重要作用。因此，微生物制造已广泛用于医药，工业和农业。但是，包括酶工程，途径优化和宿主工程在内的通用策略通常不足以获得有效的微生物生产系统。转运蛋白工程提供了一种替代策略，以促进微生物细胞中底物，中间体和最终产物的跨膜转移，从而通过减轻最终产物引起的反馈抑制和细胞毒性来提高产量。根据运输工程方面的最新研究，本地运输商通常表达较低且运输能力较差，导致运输过程效率低下和微生物产生。在这篇综述中，对运输者的采矿，表征和验证的当前方法进行了全面总结。讨论了在工程细胞中增强转运系统的实际方法，例如平衡转运蛋白的过表达和细胞生长以及天然转运蛋白的进化。此外，转运蛋白工程技术在微生物制造中的应用，包括提高底物利用率，向目标途径代谢通量的集中以及加速外排和回收产品等，均显示了其突出的优势和广阔的前景。讨论了在工程细胞中增强转运系统的实际方法，例如平衡转运蛋白的过表达和细胞生长以及天然转运蛋白的进化。此外，转运蛋白工程技术在微生物制造中的应用，包括提高底物利用率，向目标途径代谢通量的集中以及加速外排和回收产品，均显示出其突出的优势和广阔的前景。讨论了在工程细胞中增强转运系统的实际方法，例如平衡转运蛋白的过表达和细胞生长以及天然转运蛋白的进化。此外，转运蛋白工程技术在微生物制造中的应用，包括提高底物利用率，向目标途径代谢通量的集中以及加速外排和回收产品，均显示出其突出的优势和广阔的前景。

更新日期：2020-04-16

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