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Electroosmotic Facilitated Protein Capture and Transport through Solid‐State Nanopores with Diameter Larger than Length
Small Methods ( IF 10.7 ) Pub Date : 2020-04-16 , DOI: 10.1002/smtd.201900893 Yin Zhang 1 , Jiabin Zhao 1 , Wei Si 1 , Yajing Kan 1 , Zheng Xu 2 , Jingjie Sha 1 , Yunfei Chen 1
Small Methods ( IF 10.7 ) Pub Date : 2020-04-16 , DOI: 10.1002/smtd.201900893 Yin Zhang 1 , Jiabin Zhao 1 , Wei Si 1 , Yajing Kan 1 , Zheng Xu 2 , Jingjie Sha 1 , Yunfei Chen 1
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Solid‐state nanopores can be a powerful tool to investigate proteins in their native state. However, the excessive fast translocation speed of proteins causes the majority of events to remain undetected. Here, the behaviors of streptavidin (SA) transport through a solid‐state nanopore are dominated by electroosmotic flow (EOF). Experimental results show the frequency of translocation events detected by the nanopore with a diameter slightly larger than length is ≈17 times larger than that in previously reported work. Numerical simulations elucidate the enhanced frequency comes from a concave‐shape EOF in the nanopore, which provides a low water velocity region allowing for numbers of SAs moving slowly enough to be detected. However, as pore diameter increases, the maximum detectable velocity of SAs decreases due to the reduced signal‐to‐noise ratio. Moreover, it is found the SA translocation frequency slowly increases with the amplitude of voltages, and then declines at a threshold bias. This phenomenon can be attributed to the bandwidth limitation combined with the non‐linear dependence of EOF velocity in the pore central region on applied voltage.
中文翻译：
电渗促进直径大于长度的固态纳米孔的蛋白质捕获和运输
固态纳米孔可以成为研究天然状态蛋白质的强大工具。但是，蛋白质过快的快速转运速度会导致大多数事件未被发现。在这里，链霉亲和素（SA）通过固态纳米孔的传输行为主要由电渗流（EOF）决定。实验结果表明，直径稍大于长度的纳米孔检测到的移位事件的频率是以前报道的工作中≈17倍。数值模拟表明，提高的频率来自纳米孔中的凹形EOF，该EOF提供了低水速区域，允许大量SA缓慢移动以检测到。但是，随着孔径的增加，由于信噪比降低，SAs的最大可检测速度降低。而且，发现SA易位频率随着电压的幅度缓慢增加，然后在阈值偏置处下降。这种现象可归因于带宽限制以及孔中心区域EOF速度对施加电压的非线性依赖性。
更新日期：2020-04-16
中文翻译：
电渗促进直径大于长度的固态纳米孔的蛋白质捕获和运输
固态纳米孔可以成为研究天然状态蛋白质的强大工具。但是，蛋白质过快的快速转运速度会导致大多数事件未被发现。在这里，链霉亲和素（SA）通过固态纳米孔的传输行为主要由电渗流（EOF）决定。实验结果表明，直径稍大于长度的纳米孔检测到的移位事件的频率是以前报道的工作中≈17倍。数值模拟表明，提高的频率来自纳米孔中的凹形EOF，该EOF提供了低水速区域，允许大量SA缓慢移动以检测到。但是，随着孔径的增加，由于信噪比降低，SAs的最大可检测速度降低。而且，发现SA易位频率随着电压的幅度缓慢增加，然后在阈值偏置处下降。这种现象可归因于带宽限制以及孔中心区域EOF速度对施加电压的非线性依赖性。
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