Atropa acuminata aqueous leaf extract biosynthesized silver nanoparticles showed strong antioxidant, anticancerous (HeLa cells) and anti-inflammatory activities. Besides, this bio syn-AgNP also proved effective against mosquito vectors causing malaria, dengue and filariasis. Present study highlights eco-friendly and sustainable approach for the synthesis of silver nanoparticles (AgNP) using aqueous leaf extract of A. acuminata, a critically endangered medicinal herb. The addition of 1 mM silver nitrate to aqueous leaf extract resulted in the synthesis of AgNP when solution was heated at 60 °C for 30 min at pH 7. Absorption band at 428 nm, as shown by UV-Vis spectroscopy confirmed the synthesis of AgNP. XRD patterns revealed the crystalline nature of AgNP and TEM analysis showed that most of the nanoparticles were spherical in shape. Zeta potential of AgNP was found to be - 33.5 mV which confirmed their high stability. FT-IR investigations confirmed the presence of different functional groups involved in the reduction and capping of AgNP. The synthesized AgNP showed effective DPPH (IC50-16.08 µg/mL), H2O2 (IC50-25.40 µg/mL), and superoxide (IC50-21.12 µg/mL) radical scavenging activities. These plant-AgNP showed significant inhibition of albumin denaturation (IC50-12.98 µg/mL) and antiproteinase activity (IC50-18.401 µg/mL). Besides, biosynthesized AgNP were found to have strong inhibitory effect against a cervical cancer (HeLa) cell line (IC50-5.418 µg/mL) as well as larvicidal activity against 3rd instar larvae of Anopheles stephensi (LC50-18.9 ppm, LC90-40.18 ppm), Aedes aegypti (LC50-12.395 ppm, LC90-36.34 ppm) and Culex quinquefasciatus (LC50-17.76 ppm, LC90-30.82 ppm) and were found to be non-toxic against normal cell line (HEK 293), and a non-target organism (Mesocyclops thermocyclopoides). This is the first report on the synthesis of AgNP using aqueous leaf extract of A. acuminata, validating their strong therapeutic potential.

中文翻译：

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使用印度颠茄提取物绿色合成银纳米粒子及其潜在的抗氧化、抗炎、抗癌和杀幼虫活性。

Atropa acuminata 水叶提取物生物合成的银纳米粒子显示出强大的抗氧化、抗癌（HeLa 细胞）和抗炎活性。此外，这种生物 syn-AgNP 也被证明对引起疟疾、登革热和丝虫病的蚊媒有效。目前的研究强调了利用极度濒危的药草 A. acuminata 的水叶提取物合成银纳米粒子 (AgNP) 的环保和可持续方法。当溶液在 60 °C、pH 7 下加热 30 分钟时，向水叶提取物中添加 1 mM 硝酸银导致 AgNP 的合成。UV-Vis 光谱所示的 428 nm 吸收带证实了 AgNP 的合成. XRD 图案揭示了 AgNP 的结晶性质，TEM 分析表明大多数纳米颗粒是球形的。发现 AgNP 的 Zeta 电位为 - 33.5 mV，这证实了它们的高稳定性。FT-IR 研究证实了参与 AgNP 还原和封端的不同官能团的存在。合成的 AgNP 显示出有效的 DPPH (IC50-16.08 µg/mL)、H2O2 (IC50-25.40 µg/mL) 和超氧化物 (IC50-21.12 µg/mL) 自由基清除活性。这些植物-AgNP 显示出对白蛋白变性 (IC50-12.98 µg/mL) 和抗蛋白酶活性 (IC50-18.401 µg/mL) 的显着抑制。此外，发现生物合成的 AgNP 对宫颈癌 (HeLa) 细胞系具有很强的抑制作用 (IC50-5.418 µg/mL) 以及对斯蒂芬按蚊 3 龄幼虫的杀幼虫活性 (LC50-18.9 ppm, LC90-40.18 ppm) )、埃及伊蚊 (LC50-12.395 ppm, LC90-36.34 ppm) 和库蚊 (LC50-17.76 ppm, LC90-30。82 ppm）并且被发现对正常细胞系（HEK 293）和非目标生物体（Mesocyclops thermocyclopoides）无毒。这是关于使用 A. acuminata 的水叶提取物合成 AgNP 的第一份报告，验证了它们的强大治疗潜力。