The effect of Aso volcanic limonite was explored in anaerobic digestion using waste sewage sludge (WSS). In this study, methane and hydrogen sulfide were remarkably inhibited when Aso limonite was mixed with WSS as well as a significant reduction of ammonia. Although pH was lowered after adding Aso limonite, methane was still inhibited in neutralized pH condition at 7.0. Hydrolysis stage was not influenced by Aso limonite as supported by the result that a high protease activity was still detected in the presence of the material. However, acidogenesis stage was affected by Aso limonite as indicated by the different productions of organic acids. Acetic acid, was accumulated in the presence of Aso limonite due to the inhibition of methane production, except in the highest concentration of Aso limonite which the production of acetate may be inhibited. Besides, the production of propionate and butyrate reduced in accordance to the increased concentration of Aso limonite. In addition, Archaeal activity (methanogens) in WSS with Aso limonite was low in agreement with the low methane production. Thus, these results indicate that Aso limonite influences the acidogenesis and methanogenesis processes, by which the productions of methane and ammonia were inhibited. On the other hand, in the contactless of Aso limonite during the anaerobic digestion of WSS (Aso limonite was placed in the area of headspace in the vial), Aso limonite had the adsorptive ability for hydrogen sulfide from WSS, but not for methane. This contactless system of Aso limonite may be a practical means to remove hydrogen sulfide without inhibiting methane production as an important bioenergy source.

中文翻译：

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麻生褐铁矿对废水污泥厌氧消化的影响。

在使用废水污泥（WSS）进行厌氧消化中，探索了Aso火山褐铁矿的作用。在这项研究中，将Aso褐铁矿与WSS混合时，甲烷和硫化氢被显着抑制，并且氨的还原率显着降低。尽管加入Aso褐铁矿后pH降低，但在中和的pH值为7.0时甲烷仍被抑制。水解阶段不受Aso褐铁矿的影响，其结果是在存在该物质的情况下仍检测到高蛋白酶活性。然而，酸生成阶段受到麻生褐铁矿的影响，如有机酸的不同产生所表明的。由于存在抑制甲烷生成的作用，乙酸在Aso褐铁矿的存在下积累，但在最高浓度的Aso褐铁矿中，乙酸的生成可能会受到抑制。此外，随着Aso褐铁矿浓度的增加，丙酸和丁酸的产生减少。另外，与低甲烷产生量相一致的是，具有Aso褐铁矿的WSS中的古细菌活性（产甲烷菌）较低。因此，这些结果表明，Aso褐铁矿影响了酸生成和甲烷生成过程，从而抑制了甲烷和氨的产生。另一方面，在WSS厌氧消化过程中非接触式的Aso褐铁矿（将Aso褐铁矿放在小瓶顶部空间），Aso褐铁矿具有对WSS硫化氢的吸附能力，但对甲烷没有吸附能力。Aso褐铁矿的这种非接触系统可能是去除硫化氢而不抑制甲烷作为重要生物能源的一种实用方法。随着Aso褐铁矿浓度的增加，丙酸和丁酸的产量降低。另外，与低甲烷产生量相一致的是，具有Aso褐铁矿的WSS中的古细菌活性（产甲烷菌）较低。因此，这些结果表明，Aso褐铁矿影响了酸生成和甲烷生成过程，从而抑制了甲烷和氨的产生。另一方面，在WSS厌氧消化过程中非接触式的Aso褐铁矿（将Aso褐铁矿放在小瓶顶部空间），Aso褐铁矿具有对WSS硫化氢的吸附能力，但对甲烷没有吸附能力。Aso褐铁矿的这种非接触系统可能是去除硫化氢而不抑制甲烷作为重要生物能源的一种实用方法。随着Aso褐铁矿浓度的增加，丙酸和丁酸的产量降低。另外，与低甲烷产生量相一致的是，具有Aso褐铁矿的WSS中的古细菌活性（产甲烷菌）较低。因此，这些结果表明，Aso褐铁矿影响了酸生成和甲烷生成过程，从而抑制了甲烷和氨的产生。另一方面，在WSS厌氧消化过程中非接触式的Aso褐铁矿（将Aso褐铁矿放在小瓶顶部空间），Aso褐铁矿具有对WSS硫化氢的吸附能力，但对甲烷没有吸附能力。Aso褐铁矿的这种非接触系统可能是去除硫化氢而不抑制甲烷作为重要生物能源的一种实用方法。