The present study investigated the effects of CS@Fe₃O₄ nanoparticles combined with microwave or far infrared thawing on microbial diversity of red seabream (Pagrus major) fillets in terms of thawing loss, pH, TVB-N, classical microbiological enumeration and high-throughput sequencing, and the same parameters were also studied for 24 h after thawing. Four thawing methods were used: microwave thawing (MT), far-infrared thawing (FT), CS@Fe₃O₄ nanoparticles combined with microwave thawing (CMT) and CS@Fe₃O₄ nanoparticles combined with far-infrared thawing (CFT). The results showed that CFT and CMT had lower values of pH and TVB-N compared to the FT and MT. Based on conventional microbial count analysis, CFT and CMT samples also maintained lower TVC, pseudomonas and LAB counts. Using high-throughput sequencing analysis, Compared with FT and MT, CFT and CMT samples showed a significant decrease in the proportion of the Pseudomonadaceae flora. However, the proportion of Pseudomonas, Bacillaceae and Thermaceae also increased significantly after 24 h of storage, which indicated that become the predominant microbiota in red seabream (Pagrus major) fillets.

本研究探讨CS的效果@的Fe _3个ö ₄纳米颗粒与微波或对真鲷的微生物多样性（远红外解冻组合真鲷）在解冻损失，pH值，TVB-N，古典微生物枚举和方面鱼片高融化后24 h还研究了通量测序和相同的参数。使用了四种解冻方法：微波解冻（MT），远红外解冻（FT），CS @ Fe ₃ O ₄纳米粒子与微波解冻（CMT）和CS @ Fe ₃ O _4结合纳米粒子结合远红外解冻（CFT）。结果表明，与FT和MT相比，CFT和CMT具有较低的pH和TVB-N值。根据常规微生物计数分析，CFT和CMT样品也保持较低的TVC，假单胞菌和LAB计数。使用高通量测序分析，与FT和MT相比，CFT和CMT样品显示假单孢菌科菌群的比例显着降低。然而，在储存24小时后，假单胞菌，杆菌科和菊科的比例也显着增加，这表明其成为红鲷鱼（Pagrus major）鱼片中的主要微生物群。