Background In this study, renewable tea waste hydrolysate was used as a sole carbon source for carotenoids and lipid production. A novel Rhodosporidium toruloides mutant strain, RM18, was isolated through atmospheric and room-temperature plasma mutagenesis and continuous domestication in tea waste hydrolysate from R. toruloides ACCC20341. Results RM18 produced a larger biomass and more carotenoids and α-linolenic acid compared with the control strain cultured in tea waste hydrolysate. The highest yields of torularhodin (481.92 μg/g DCW) and torulene (501 μg/g DCW) from RM18 cultured in tea waste hydrolysate were 12.86- and 1.5-fold higher, respectively, than that of the control strain. In addition, α-linolenic acid production from RM18 in TWH accounted for 5.5% of total lipids, which was 1.58 times more than that of the control strain. Transcriptomic profiling indicated that enhanced central metabolism and terpene biosynthesis led to improved carotenoids production, whereas aromatic amino acid synthesis and DNA damage checkpoint and sensing were probably relevant to tea waste hydrolysate tolerance. Conclusion Tea waste is suitable for the hydrolysis of microbial cell culture mediums. The R. toruloides mutant RM18 showed considerable carotenoids and lipid production cultured in tea waste hydrolysate, which makes it viable for industrial applications.

中文翻译：

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茶渣水解液中培养的圆红冬孢酵母的类胡萝卜素和脂质生产。


背景在这项研究中，可再生茶渣水解产物被用作类胡萝卜素和脂质生产的唯一碳源。通过常压和室温等离子体诱变以及在茶渣水解产物中持续驯化，从圆红冬孢菌 ACCC20341 中分离出一种新的圆红冬孢酵母突变株 RM18。结果与茶渣水解液中培养的对照菌株相比，RM18产生了更大的生物量、更多的类胡萝卜素和α-亚麻酸。在茶渣水解液中培养的 RM18 中，圆环菌素 (481.92 μg/g DCW) 和 torulene (501 μg/g DCW) 的最高产量分别比对照菌株高 12.86 倍和 1.5 倍。此外，TWH中RM18的α-亚麻酸产量占总脂质的5.5%，是对照菌株的1.58倍。转录组分析表明，中枢代谢和萜烯生物合成的增强导致类胡萝卜素产量的提高，而芳香氨基酸合成和 DNA 损伤检查点和传感可能与茶渣水解产物的耐受性相关。结论茶渣适合微生物细胞培养基的水解。 R. toruloides 突变体 RM18 在茶渣水解产物中培养时显示出大量的类胡萝卜素和脂质产量，这使其具有工业应用的可行性。