Gill parasitic infections challenge farming of rainbow trout (Oncorhynchus mykiss, Walbaum) in freshwater facilities. Apart from flagellates (Ichthyobodo, (Pinto) and ciliates (Ichthyophthirius (Fouquet), Ambiphrya (Raabe), Apiosoma (Blanchard), Trichodinella (Sramek‐Husek) and Trichodina (Ehrenberg)), we have shown that amoebae are prevalent in Danish trout farms. Gills were isolated from farmed rainbow trout in six fish farms (conventional and organic earth pond and recirculated systems) and placed on non‐nutrient agar (NNA) moistened with modified Neff's amoeba saline (AS) (15°C). Gill amoebae from all examined fish colonized the agar and were identified based on morphological criteria showing species within the genera Trinema (Dujardin) (family Trinematidae), Vannella (Bovee) (family Vannellidae). In addition, hartmannellid amoebae were recorded. We established a monoculture of Vannella sp., confirmed the genus identity by PCR and sequencing and performed an in vitro determination of antiparasitic effects (dose–response studies) of various compounds including sodium chloride (NaCl), hydrogen peroxide, peracetic acid, formalin, aqueous garlic and oregano extracts and a Pseudomonas H6 surfactant. All amoebae were killed in concentrations of 16.90 mg/ml (garlic), 17.90 mg/ml (oregano), NaCl (7.5 mg/ml), hydrogen peroxide (100 µg/ml), peracetic acid (0.03 µg/ml), formaldehyde (25 µg/ml) and the Pseudomonas H6 surfactant (250 µg/ml).

中文翻译：

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淡水虹鳟（Oncorhynchus mykiss）的ill虫：对Vannella sp。的抗寄生虫化合物的体外评估。

ill寄生虫感染挑战了在淡水设施中养殖虹鳟鱼（Oncorhynchus mykiss， Walbaum）。除了鞭毛虫（Ichthyobodo， （托）和纤毛虫（小瓜（富凯），Ambiphrya（拉贝），Apiosoma（查德），Trichodinella（Sramek-Husek）和车轮虫（Ehrenberg）），我们已经证明变形虫在丹麦鳟鱼养殖场中很普遍。在六个养鱼场（常规和有机土池和循环系统）中，从养殖的虹鳟鱼中分离出G，然后放在用改良的内夫氏变形虫盐水（AS）（15℃）润湿的非营养琼脂（NNA）上。所有受检鱼的变形虫a都在琼脂上定居，并根据形态学标准进行鉴定，该形态标准显示了Trinema（Dujardin）（Trinematidae科），Vannella（Bovee）（Vannellidae）科内的物种。此外，还记录了哈氏杆菌。我们建立了Vannella的单一文化等通过PCR和测序确认属属，并进行了体外测定各种化合物（包括氯化钠（NaCl），过氧化氢，过乙酸，福尔马林，大蒜和牛至提取物）的抗寄生虫作用（剂量-反应研究）。一个假单胞菌H6表面活性剂。杀死所有变形虫的浓度分别为16.90 mg / ml（大蒜），17.90 mg / ml（牛至），NaCl（7.5 mg / ml），过氧化氢（100 µg / ml），过乙酸（0.03 µg / ml），甲醛（25 µg / ml）和假单胞菌H6表面活性剂（250 µg / ml）。