The mixotrophic cultivation of Chromochloris zofingiensis (Chlorophyta, Trebouxiophyceae) under various urea concentrations was investigated for biomass and pigment production. The results showed that urea was favorable for biomass production with the highest biomass concentration of 8.86 g L⁻¹ at 3.81 mmol L⁻¹ of urea. When urea concentration was lowered to 0.19 mmol L⁻¹, the highest content (0.99 mg g⁻¹) and yield (4.11 mg L⁻¹) of total carotenoids was reached due to an extremely high C/N ratio. Principle component analysis revealed that urea concentrations could indeed affect biomass production and pigment biosynthesis involving intracellular nitrogen assimilation and catabolism associated with chloroplast degradation. Our present study evaluates the feasibility of using urea for C. zofingiensis cultivation and provides new insights for a better understanding of the close relationships between urea metabolism and pigment biosynthesis in mixotrophic C. zofingiensis.

研究了在不同尿素浓度下的铜绿绿藻（Chlormochloris zofingiensis）（绿藻，拟南芥）的混合营养培养，以生产生物量和色素。结果表明，尿素以3.81 mmol L ^-1的最高生物质浓度为8.86 g L ^-1有利于生物质的生产。当尿素浓度降低至0.19 mmol L ^-1时，最高含量（0.99 mg g ^-1）和产量（4.11 mg L ^-1）由于极高的C / N比，总类胡萝卜素达到）。主成分分析表明，尿素浓度的确可能影响生物量的产生和色素的生物合成，涉及细胞内氮的同化作用以及与叶绿体降解有关的分解代谢。我们的研究评估了用尿素进行Zofingiensis栽培的可行性，并为更好地了解混合营养型C. zofingiensis的尿素代谢与色素生物合成之间的密切关系提供了新的见识。