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Network Pharmacological Screening of the Active Ingredients and Hypoglycemic Effect of Isodon rubescens in the Treatment of Diabetes
Planta Medica ( IF 2.1 ) Pub Date : 2020-04-15 , DOI: 10.1055/a-1147-9196
Xue Jintao 1 , Yu Shasha 1 , Wang Jincai 2 , Li Chunyan 1, 3 , Yang Mengya 1 , Shi Yongli 1
Affiliation  

This study was firstly to study the relationship of "ingredient-target-pathway" and the pharmacological effects of Isodon rubescens for the treatment of diabetes. Based on a network pharmacology method, 138 active ingredients of Isodon rubescens were screened from the relative literatures, and their targets were confirmed by comparing these with the hypoglycemic targets in the DrugBank database. Results showed that Isodon rubescens contained 25 hypoglycemic ingredients, such as rabdoternin A, rabdoternin B, and epinodosinol. These ingredients could activate 6 hypoglycemic targets, including 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGCR), integrin α-L (ITGAL), integrin β-2 (ITGB2), progesterone receptor (PGR), glucocorticoid receptor (NR3C1), and nuclear receptor subfamily 1 group I member 2 (NR1I2). These targets were involved in 94 signaling pathways, such as the Rap1, PI3K-Akt, and HIF-1 signaling pathways. The cell viability showed that the human umbilical vein endothelial cells (HUVECs) treated with alcohol extract (1.00 g/L) and the water extract (0.13 - 0.50 g/L) exhibited high viability compared to the model group (p < 0.05), respectively. 0In animal experiments, the rats treated with water extract of Isodon rubescens showed significant hypoglycemic effects compared to rats in the model group (p < 0.05). Overall, this approach provides an efficient strategy to explore hypoglycemic ingredients of Isodon rubescens and other traditional Chinese medicine.

中文翻译:

冬虫夏草有效成分的网络药理筛选及降糖作用治疗糖尿病

本研究首先研究了“成分-靶点-通路”与冬凌草治疗糖尿病的药理作用的关系。基于网络药理学方法,从相关文献中筛选出138种冬凌草有效成分,并与DrugBank数据库中的降糖靶点进行比较,确定其靶点。结果表明,冬凌草含有25种降血糖成分,如rabdoternin A、rabdoternin B和epinodosinol。这些成分可以激活 6 个降糖靶点,包括 3-羟基-3-甲基戊二酰辅酶 A 还原酶 (HMGCR)、整合素 α-L (ITGAL)、整合素 β-2 (ITGB2)、孕酮受体 (PGR)、糖皮质激素受体 (NR3C1) ) 和核受体亚家族 1 组 I 成员 2 (NR1I2)。这些靶标参与了 94 条信号通路,例如 Rap1、PI3K-Akt 和 HIF-1 信号通路。细胞活力显示,与模型组相比,用酒精提取物(1.00 g/L)和水提取物(0.13 - 0.50 g/L)处理的人脐静脉内皮细胞(HUVECs)表现出较高的活力(p < 0.05),分别。0在动物实验中,冬虫夏草水提物处理的大鼠与模型组大鼠相比,显示出显着的降血糖作用(p < 0.05)。总体而言,该方法为探索冬凌草等中药的降血糖成分提供了有效的策略。细胞活力显示,与模型组相比,用酒精提取物(1.00 g/L)和水提取物(0.13 - 0.50 g/L)处理的人脐静脉内皮细胞(HUVECs)表现出较高的活力(p < 0.05),分别。0在动物实验中,冬虫夏草水提物处理的大鼠与模型组大鼠相比,显示出显着的降血糖作用(p < 0.05)。总体而言,该方法为探索冬凌草等中药的降血糖成分提供了有效的策略。细胞活力显示,与模型组相比,用酒精提取物(1.00 g/L)和水提取物(0.13 - 0.50 g/L)处理的人脐静脉内皮细胞(HUVECs)表现出较高的活力(p < 0.05),分别。0在动物实验中,冬虫夏草水提物处理的大鼠与模型组大鼠相比,显示出显着的降血糖作用(p < 0.05)。总体而言,该方法为探索冬凌草等中药的降血糖成分提供了有效的策略。与模型组大鼠相比,冬凌草水提物处理的大鼠显示出显着的降血糖作用(p < 0.05)。总体而言,该方法为探索冬凌草等中药的降血糖成分提供了有效的策略。与模型组大鼠相比,冬凌草水提物处理的大鼠显示出显着的降血糖作用(p < 0.05)。总体而言,该方法为探索冬凌草等中药的降血糖成分提供了有效的策略。
更新日期:2020-04-15
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