Root-knot nematode (RKN) disease is a soil-borne disease. However, most studies on RKN have focused on the screening of agents and the cultivation of resistant varieties, and reports on the interaction of RKNs with soil microorganisms are few. In this study, we performed Illumina high-throughput sequencing to analyze diseased and healthy soil and the microbial-community changes in rhizosphere soil after microbial treatment (Pseudomonas flurescens, Bacillus subtilis, Paecolomyces lilacinus). Results showed significant differences in the bacterial community richness and diversity between diseased and healthy soil and the presence of different microbial species. After treatment, the richness and diversity of microbial communities in soil, as well as the number and incidence of second-stage juvenile of RKNs, decreased. Through linear discriminant analysis effect size, Pearson correlation, and Venn diagram analysis, we screened five genera that were closely related to disease occurrence, among which Pseudomonas was most related to disease inhibition. Our results suggested that the occurrence of tobacco RKN was related to changes in soil microbial communities, and that the interactions among Pseudomonas, Bryobacter, Variibacter, Coniochaeta, and Metarhizium affected the health of rhizosphere soil.

中文翻译：

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探索影响根系线虫线虫根际的关键微生物变化。

根结线虫（RKN）病是一种土壤传播的疾病。然而，大多数关于RKNs的研究都集中在筛选病原和抗病品种的培养上，关于RKNs与土壤微生物相互作用的报道很少。在这项研究中，我们进行了Illumina高通量测序，以分析患病和健康的土壤以及经过微生物处理（假单胞菌，枯草芽孢杆菌，淡紫色拟青霉菌）后的根际土壤微生物群落变化。结果表明，患病土壤和健康土壤之间以及不同微生物物种的存在下，细菌群落的丰富度和多样性存在显着差异。处理后，土壤微生物群落的丰富性和多样性以及RKNs二级幼虫的数量和发生率均下降。通过线性判别分析效应大小，Pearson相关性和Venn图分析，我们筛选了与疾病发生密切相关的五个属，其中假单胞菌与疾病抑制最相关。我们的结果表明，烟草RKN的发生与土壤微生物群落的变化有关，并且假单胞菌，假单胞菌，变异杆菌，Coniochaeta和金属根之间的相互作用影响了根际土壤的健康。