Ocean acidification alters physiology, acid-base balance and metabolic activity in marine animals. Near future elevated pCO₂ conditions could be expected to influence the bioaccumulation of metals, feeding rate and immune parameters in marine mussels. To better understand such impairments, a series of laboratory-controlled experiment was conducted by using a model marine mussel, Mytilus galloprovincialis. The mussels were exposed to three pH conditions according to the projected CO₂ emissions in the near future (one ambient: 8.10 and two reduced: 7.80 and 7.50). At first, the bioconcentration of Ag and Cd was studied in both juvenile (2.5 cm) and adult (5.1 cm) mussels by using a highly sensitive radiotracer method (^110mAg and ¹⁰⁹Cd). The uptake and depuration kinetics were followed 21 and 30 days, respectively. The biokinetic experiments demonstrated that the effect of ocean acidification on bioconcentration was metal-specific and size-specific. The uptake, depuration and tissue distribution of ^110mAg were not affected by elevated pCO₂ in both juvenile and adult mussels, whereas ¹⁰⁹Cd uptake significantly increased with decreasing pH in juveniles but not in adults. Regardless of pH, ^110mAg accumulated more efficiently in juvenile mussels than adult mussels. After executing the biokinetic experiment, the perturbation was sustained by using the same mussels and the same experimental set-up, which enabled us to determine filtration rate, haemocyte viability, lysosomal membrane stability, circulating cell-free nucleic acids (ccf-NAs) and protein (ccf-protein) levels. The filtration rate and haemocyte viability gradually decreased by increasing pCO₂ level, whereas the lysosomal membrane stability, ccf-NAs, and ccf-protein levels remained unchanged in the mussels exposed to elevated pCO₂ for eighty-two days. This study suggests that acidified seawater partially shift metal bioaccumulation, physiological and cellular parameters in the mussel Mytilus galloprovincialis.

海洋酸化改变了海洋动物的生理，酸碱平衡和代谢活性。预计在不久的将来升高的p CO ₂条件会影响海洋贻贝中金属的生物积累，进食速率和免疫参数。为了更好地理解此类损害，使用模型海洋贻贝Mytilus galloprovincialis进行了一系列实验室控制的实验。根据近期内预计的CO ₂排放量，贻贝处于三种pH条件下（一种环境：8.10，两种减少：7.80和7.50）。首先，通过使用高灵敏度放射性示踪剂方法（^110m）研究了贻贝（2.5 cm）和成年贻贝（5.1 cm）中银和镉的生物浓度。Ag和¹⁰⁹ Cd）。分别跟踪摄取和净化动力学21和30天。生物动力学实验表明，海洋酸化对生物浓度的影响是金属特异性和尺寸特异性的。在幼贻贝和成年贻贝中，p CO ₂的升高均不会影响^110m Ag的吸收，净化和组织分布，而随着成年pH值的降低，成年贻贝的¹⁰⁹ Cd吸收显着增加，而成年贻贝则没有。不论pH值为^110m银在贻贝中的积累比成年贻贝更有效。在执行生物动力学实验后，使用相同的贻贝和相同的实验装置维持了摄动，这使我们能够确定滤过率，血细胞活力，溶酶体膜稳定性，循环性无细胞核酸（ccf-NAs）和蛋白质（ccf-蛋白质）含量。通过增加p CO ₂水平，滤过率和血细胞存活力逐渐降低，而暴露于p CO ₂升高的贻贝中的溶酶体膜稳定性，ccf-NAs和ccf-蛋白质水平保持不变。八十二天。这项研究表明，酸化的海水会部分改变贻贝贻贝贻贝中金属的生物积累，生理和细胞参数。