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Extracellular vesicles spread the RNA interference signal of Tribolium castaneum TcA cells.
Insect Biochemistry and Molecular Biology ( IF 3.2 ) Pub Date : 2020-04-14 , DOI: 10.1016/j.ibmb.2020.103377 Lina Mingels 1 , Niels Wynant 1 , Dulce Santos 1 , Paulien Peeters 1 , Yannick Gansemans 2 , Johan Billen 3 , Filip Van Nieuwerburgh 2 , Jozef Vanden Broeck 1
Insect Biochemistry and Molecular Biology ( IF 3.2 ) Pub Date : 2020-04-14 , DOI: 10.1016/j.ibmb.2020.103377 Lina Mingels 1 , Niels Wynant 1 , Dulce Santos 1 , Paulien Peeters 1 , Yannick Gansemans 2 , Johan Billen 3 , Filip Van Nieuwerburgh 2 , Jozef Vanden Broeck 1
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The potential utility of RNA interference (RNAi) to control insect pests and viral infections depends largely on the target organism's ability to systemically spread the RNAi response. The efficacy of systemic RNAi varies among insects, though it has been shown to be high in the red flour beetle, Tribolium castaneum. We identified an extracellular RNAi signal that is present in the culture medium of T. castaneum (TcA) cells after treatment with long dsRNA specific for a luciferase reporter gene. Luciferase-specific siRNAs were detected in extracellular vesicles (EVs) that were purified from the culture medium of these dsRNA-treated cells. Furthermore, by measuring the silencing of luciferase expression, we showed that these siRNA-containing EVs can act as an RNAi signal for recipient TcA cells. We have therefore shown that a systemic RNAi response upon dsRNA treatment can be effectively spread through EVs.
中文翻译：
细胞外囊泡传播了Tribolium castaneum TcA细胞的RNA干扰信号。
RNA干扰（RNAi）用于控制害虫和病毒感染的潜在效用在很大程度上取决于目标生物体系统性传播RNAi反应的能力。全身性RNAi的功效因昆虫而异，尽管在红色面粉甲虫Tribolium castaneum中已显示出很高的功效。我们确定了一种特定于萤光素酶报道基因的长dsRNA处理后，在锥栗（TcA）细胞培养基中存在的胞外RNAi信号。在从这些dsRNA处理的细胞的培养基中纯化的细胞外囊泡（EV）中检测到萤光素酶特异性siRNA。此外，通过测量荧光素酶表达的沉默，我们表明这些含siRNA的电动汽车可以充当受体TcA细胞的RNAi信号。
更新日期：2020-04-14
中文翻译：
细胞外囊泡传播了Tribolium castaneum TcA细胞的RNA干扰信号。
RNA干扰（RNAi）用于控制害虫和病毒感染的潜在效用在很大程度上取决于目标生物体系统性传播RNAi反应的能力。全身性RNAi的功效因昆虫而异，尽管在红色面粉甲虫Tribolium castaneum中已显示出很高的功效。我们确定了一种特定于萤光素酶报道基因的长dsRNA处理后，在锥栗（TcA）细胞培养基中存在的胞外RNAi信号。在从这些dsRNA处理的细胞的培养基中纯化的细胞外囊泡（EV）中检测到萤光素酶特异性siRNA。此外，通过测量荧光素酶表达的沉默，我们表明这些含siRNA的电动汽车可以充当受体TcA细胞的RNAi信号。
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