Depletion of microglia and macrophages with clodronate liposomes attenuates zymosan-induced Fos expression and hypothermia in the adult mouse,Journal of Neuroimmunology

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Depletion of microglia and macrophages with clodronate liposomes attenuates zymosan-induced Fos expression and hypothermia in the adult mouse
Journal of Neuroimmunology ( IF 2.9 ) Pub Date : 2020-07-01 , DOI: 10.1016/j.jneuroim.2020.577244
Shohei Takagi ₁ , Saki Murayama ₁ , Katsuhiro Torii ₁ , Shoko Takemura-Morita ₂ , Erkin Kurganov ₁ , Sumiharu Nagaoka ₁ , Akio Wanaka ₂ , Seiji Miyata ₁

Affiliation

Toll-like receptor 2 (TLR2) recognizes a wide range of microbial molecules and plays critical roles in the initiation of innate immune responses. In the present study, we aimed to investigate whether the depletion of microglia and macrophages with clodronate liposomes (Clod-Lips) attenuates the activation of mouse brain circuits for TLR2-mediated inflammation and hypothermia. The peripheral administration of the TLR2 agonist zymosan induced nuclear factor-κB activation in microglia and macrophages and Fos expression in astrocytes/tanycytes and neurons in the circumventricular organs (CVOs). The depletion of microglia and macrophages with Clod-Lips markedly decreased zymosan-induced Fos expression in astrocytes/tanycytes and neurons in the CVOs. The treatment with Clod-Lips significantly attenuated zymosan-induced hypothermia. These results indicate that microglia and macrophages in the CVOs participate in the initiation and transmission of inflammatory responses after the peripheral administration of zymosan.

中文翻译：

用氯膦酸盐脂质体去除小胶质细胞和巨噬细胞可减弱酵母聚糖诱导的成年小鼠 Fos 表达和体温过低

Toll 样受体 2 (TLR2) 可识别多种微生物分子，并在先天免疫反应的启动中发挥关键作用。在本研究中，我们旨在研究用氯膦酸脂质体 (Clod-Lips) 消耗小胶质细胞和巨噬细胞是否会减弱小鼠脑回路的激活，从而导致 TLR2 介导的炎症和体温过低。TLR2 激动剂酵母聚糖的外周给药诱导小胶质细胞和巨噬细胞中的核因子-κB 活化，以及环室器官 (CVO) 中星形胶质细胞/长链细胞和神经元中的 Fos 表达。用Clod-Lips 消耗小胶质细胞和巨噬细胞显着降低了酵母聚糖诱导的CVO 中星形胶质细胞/tanycytes 和神经元中的Fos 表达。用Clod-Lips 处理显着减轻酵母聚糖引起的体温过低。

更新日期：2020-07-01

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