Abstract Microtransplantation of rat brain neurolemma into the plasma membrane of Xenopus laevis oocytes is an ex vivo method used to study channels and receptors in their native state using standard electrophysiological approaches. In this review, we show that oocytes injected with adult rat brain neurolemma elicited tetrodotoxin-sensitive inward ion currents upon membrane depolarization, which were increased in a concentration-dependent manner by treatment with the pyrethroid insecticides permethrin and deltamethrin. Under our initial protocols, oocyte health was reduced over time and neurolemma incorporation varied between batches of oocytes from different frogs, limiting the usefulness of the assay for regulatory issues. A collection of changes to the assay procedure, data acceptance criteria, and analysis method yield substantially improved precision and, hence, assay performance. These changes established this ex vivo approach as a toxicologically relevant assay to study the toxicodynamic action of pyrethroids on ion channels in their native state using neurolemma fragments prepared from juvenile and adult rat brains.

中文翻译：

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Neurolemma 注射非洲爪蟾卵母细胞：研究拟除虫菊酯对其天然状态离子通道影响的创新体外方法

摘要 将大鼠脑神经膜微移植到非洲爪蟾卵母细胞的质膜中是一种离体方法，用于使用标准电生理学方法研究天然状态下的通道和受体。在这篇综述中，我们展示了注射成年大鼠脑神经膜的卵母细胞在膜去极化时引起河豚毒素敏感的内向离子电流，通过用拟除虫菊酯杀虫剂氯菊酯和溴氰菊酯处理以浓度依赖性方式增加。在我们最初的协议下，卵母细胞的健康随着时间的推移而降低，神经膜的结合在来自不同青蛙的卵母细胞批次之间有所不同，限制了该检测对监管问题的有用性。对化验程序、数据接受标准、和分析方法显着提高了精度，从而提高了测定性能。这些变化确立了这种离体方法作为毒理学相关的分析方法，用于研究拟除虫菊酯在天然状态下对离子通道的毒理学作用，使用从幼年和成年大鼠大脑制备的神经膜碎片。