Nowadays the advent of innovative high-throughput sequencing allows obtaining high-quality microbiome profiling. However, PCR-based tests are still considered the "golden standard" for many clinical applications. Here, we designed a qPCR-based platform with fluorescent-labeled oligonucleotide probes for assessing human gut microbiome composition. The system allows conducting qualitative and semiquantitative analysis for 12 prokaryotic taxa that are prevalent in the human gut and associated with diseases, diet, age and other factors. The platform was validated by comparing microbiome profile data obtained with two different methods - the platform and high-throughput 16S rRNA sequencing - across 42 stool samples. The test can form the basis for precise and cost-efficient microbiome assay for large-scale surveys including clinical trials with interventions related to diet and disease risks.

中文翻译：

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开发带有探针的 qPCR 平台，用于量化普遍存在的和生物医学相关的人类肠道微生物类群。

如今，创新高通量测序的出现使得获得高质量的微生物组分析成为可能。然而，基于 PCR 的测试仍然被认为是许多临床应用的“黄金标准”。在这里，我们设计了一个基于 qPCR 的平台，带有荧光标记的寡核苷酸探针，用于评估人类肠道微生物组的组成。该系统允许对 12 种在人类肠道中普遍存在并与疾病、饮食、年龄和其他因素相关的原核生物分类群进行定性和半定量分析。通过比较使用两种不同方法（平台和高通量 16S rRNA 测序）在 42 个粪便样本中获得的微生物组概况数据来验证该平台。