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Cofactor NAD(P)H Regeneration: How Selective Are the Reactions?
Trends in Chemistry ( IF 14.0 ) Pub Date : 2020-04-14 , DOI: 10.1016/j.trechm.2020.03.002 Joseph W.H. Burnett , Russell F. Howe , Xiaodong Wang
中文翻译:
辅因子NAD(P)H再生:反应的选择性如何?
更新日期:2020-04-14
Trends in Chemistry ( IF 14.0 ) Pub Date : 2020-04-14 , DOI: 10.1016/j.trechm.2020.03.002 Joseph W.H. Burnett , Russell F. Howe , Xiaodong Wang
The regeneration of NAD(P)H can be achieved via catalysis, but the unequivocal determination of the species involved has not yet been fully realized. The current analytical methods of reactant/product analysis based on UV-vis spectroscopy, enzymatic assays, NMR spectroscopy, and HPLC are critically examined here with suggestions for future development.
中文翻译:
辅因子NAD(P)H再生:反应的选择性如何?
NAD(P)H的再生可通过催化来实现,但尚未完全明确地确定所涉及的物种。本文对目前基于紫外线-可见光谱,酶法测定,NMR光谱和HPLC的反应物/产物分析的分析方法进行了严格审查,并提出了对未来发展的建议。