Background Despite its high market potential, bio-based succinic acid production experienced recently a declining trend because the initial investments did not meet the expectations for rapid market growth. Thus, reducing the succinic acid production cost is imperative to ensure industrial implementation. Results Succinic acid production has been evaluated using hydrolysates from the organic fraction of municipal solid waste (OFMSW) collected from MSW treatment plants. A tailor-made enzymatic cocktail was used for OFMSW hydrolysate production containing up to 107.3 g/L carbon sources and up to 638.7 mg/L free amino nitrogen. The bacterial strains Actinobacillus succinogenes and Basfia succiniciproducens were evaluated for succinic acid production with the latter strain being less efficient due to high lactic acid production. Batch A. succinogenes cultures supplemented with 5 g/L yeast extract and 5 g/L MgCO3 reached 29.4 g/L succinic acid with productivity of 0.89 g/L/h and yield of 0.56 g/g. Continuous cultures at dilution rate of 0.06 h-1 reached 21.2 g/L succinic acid with yield of 0.47 g/g and productivity of 1.27 g/L/h. Downstream separation and purification of succinic acid was achieved by centrifugation, treatment with activated carbon, acidification with cation exchange resins, evaporation and drying, reaching more than 99% purity. Preliminary techno-economic evaluation has been employed to evaluate the profitability potential of bio-based succinic acid production. Conclusions The use of OFMSW hydrolysate in continuous cultures could lead to a minimum selling price of 2.5 $/kg at annual production capacity of 40,000 t succinic acid and OFMSW hydrolysate production cost of 25 $/t sugars.

中文翻译：

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城市固体废物的有机部分作为琥珀酸生产的可再生原料的评价。

背景 尽管生物基琥珀酸的市场潜力很大，但由于最初的投资没有达到市场快速增长的预期，生物基琥珀酸的生产最近出现了下降趋势。因此，降低丁二酸生产成本是确保工业化实施的必要条件。结果 使用从 MSW 处理厂收集的城市固体废物 (OFMSW) 的有机部分的水解产物评估了琥珀酸的生产。一种特制的酶混合物用于 OFMSW 水解产物的生产，其碳源含量高达 107.3 g/L，游离氨基氮含量高达 638.7 mg/L。对细菌菌株 Actinobacillus succinogenes 和 Basfia succiniciproducens 进行了琥珀酸生产评估，后一种菌株由于乳酸产量高而效率较低。批次 A。添加 5 g/L 酵母提取物和 5 g/L MgCO3 的 succinogenes 培养物达到 29.4 g/L 琥珀酸，产率为 0.89 g/L/h，产率为 0.56 g/g。以 0.06 h-1 的稀释率​​连续培养达到 21.2 g/L 的琥珀酸，产量为 0.47 g/g，产率为 1.27 g/L/h。琥珀酸下游分离提纯采用离心、活性炭处理、阳离子交换树脂酸化、蒸发干燥，纯度达到99%以上。初步技术经济评估已用于评估生物基琥珀酸生产的盈利潜力。结