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Comparative immunohistological study on using capsaicin, piperine, and okadaic acid for the transepithelial passage of the inactivated viral and bacterial vaccines in fish.
Microscopy Research and Technique ( IF 2.0 ) Pub Date : 2020-04-13 , DOI: 10.1002/jemt.23491 Alkhateib Y Gaafar 1 , Hirofumi Yamashita 2 , Indah Istiqomah 3 , Yasuhiko Kawato 4 , Kanae Ninomiya 5 , Abdelgayed Younes 1 , Toshihiro Nakai 5
Microscopy Research and Technique ( IF 2.0 ) Pub Date : 2020-04-13 , DOI: 10.1002/jemt.23491 Alkhateib Y Gaafar 1 , Hirofumi Yamashita 2 , Indah Istiqomah 3 , Yasuhiko Kawato 4 , Kanae Ninomiya 5 , Abdelgayed Younes 1 , Toshihiro Nakai 5
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The practical difficulty of parenteral application of fish vaccines against devastating fish diseases diverted the interest toward oral vaccination. Search for effective methods to enhance the oral uptake of viral and bacterial vaccines is continuing. The current research focus on a new role of mucosal fish vaccine adjuvants inducing the antigen uptake by enhancing vascularity or increasing intestinal permeability. Some inflammatory substances cause reversible pathology to the intestinal epithelium, which could be employed for the transepithelial passage of vaccine particles. The natural inflammatory substances used were capsaicin, piperine, and okadaic acid as 1 mg, 2 mg, and 1 μg/fish, respectively. Two inactivated vaccines were used as antigens to test the effect of these inflammatory substances in two different fish hosts. Tested vaccines were inactivated redspotted grouper nervous necrosis virus vaccine in sevenband grouper (Epinephelus septemfasciatus) and inactivated Edwardsiella tarda vaccine in red sea bream (Pagrus major) fish models. The inflammatory substances and each vaccine were anally intubated to fish. Capsaicin proved to be effectively aiding the transepithelial passage of vaccine particles more than piperine, while okadaic acid had no detectable effect.
中文翻译：
使用辣椒素，胡椒碱和冈田酸使鱼类灭活病毒和细菌疫苗经上皮通过的比较免疫组织学研究。
肠胃外应用鱼疫苗对抗毁灭性鱼类疾病的实际困难使人们对口服疫苗的兴趣转移了。寻找有效方法以增强口服病毒和细菌疫苗的摄入仍在继续。当前的研究集中在粘膜鱼疫苗佐剂通过增强血管或增加肠道通透性诱导抗原摄取的新作用。一些炎性物质引起肠上皮的可逆病理，可用于疫苗颗粒经上皮通过。所使用的天然炎症物质分别是辣椒素，胡椒碱和冈田酸，分别为每鱼1 mg，2 mg和1μg。使用两种灭活疫苗作为抗原来测试这些炎症物质在两种不同鱼类宿主中的作用。Epinephelus septemfasciatus（Epinephelus septemfasciatus）和灭活的Edwardsiella tarda疫苗在红鲷鱼（Pagrus major）鱼模型中。将发炎性物质和每种疫苗从肛门插管到鱼上。辣椒素被证明比胡椒碱更有效地帮助疫苗颗粒通过上皮，而冈田酸则没有可检测的作用。
更新日期：2020-04-13
中文翻译：
使用辣椒素，胡椒碱和冈田酸使鱼类灭活病毒和细菌疫苗经上皮通过的比较免疫组织学研究。
肠胃外应用鱼疫苗对抗毁灭性鱼类疾病的实际困难使人们对口服疫苗的兴趣转移了。寻找有效方法以增强口服病毒和细菌疫苗的摄入仍在继续。当前的研究集中在粘膜鱼疫苗佐剂通过增强血管或增加肠道通透性诱导抗原摄取的新作用。一些炎性物质引起肠上皮的可逆病理，可用于疫苗颗粒经上皮通过。所使用的天然炎症物质分别是辣椒素，胡椒碱和冈田酸，分别为每鱼1 mg，2 mg和1μg。使用两种灭活疫苗作为抗原来测试这些炎症物质在两种不同鱼类宿主中的作用。Epinephelus septemfasciatus（Epinephelus septemfasciatus）和灭活的Edwardsiella tarda疫苗在红鲷鱼（Pagrus major）鱼模型中。将发炎性物质和每种疫苗从肛门插管到鱼上。辣椒素被证明比胡椒碱更有效地帮助疫苗颗粒通过上皮，而冈田酸则没有可检测的作用。
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