Characterization of the genetic environment of blaKPC in Escherichia coli isolates from hospitals in China.,FEMS Microbiology Letters

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Characterization of the genetic environment of blaKPC in Escherichia coli isolates from hospitals in China.
FEMS Microbiology Letters ( IF 2.1 ) Pub Date : 2020-04-14 , DOI: 10.1093/femsle/fnaa064
Xuejing Yang ₁ , Yan Qi ₂ , Guoping Li ₃ , Yuying Wang ₂ , Zhengqing Lou ₂ , Yan Jiang ₄

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Carbapenem resistance in Enterobacteriaceae members has become a major challenge, and the genetic environment of bla_KPC, encoding Klebsiella pneumoniae carbapenemases, has not been fully clarified in China. In this study, we aimed to explore the genetic environment of bla_KPC in 25 carbapenem-resistant E. coli isolates from hospitals in Hangzhou Province, China. Antimicrobial susceptibility against 22 common antimicrobial agents was tested. Polymerase chain reaction (PCR) analysis was performed for screening of the resistent genes, such as bla_KPC, bla_CTX-M, bla_TEM, bla_SHV, bla_NDM, qnrA, qnrB, qnrS, aac(6’)-Ib, armA and rmtB. The genetic environment of bla_KPC were determinedin one isolate. bla_KPC was detected by PCR in all the clinical E. coli isolates. There were no strains carrying bla_NDM, qnrA, and armA. The genetic environment of bla_KPC showed that bla_KPC dissemination is plasmid mediated and that it is located in the Tn3–Tn4401 transposon complex. Encoding of bla_KPC-2 was responsible for carbapenem resistance in the 25 E. coli isolates. The genetic environment of bla_KPC was characterized by the Tn3–Tn4401 complex. Our findings may provide a theoretical basis for clinical drug-resistance monitoring, anti-infection treatment, and hospital infection control.

更新日期：2020-04-14

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