Spatial heterogeneity of oxygenation and haemodynamics in breast cancer resolved in vivo by conical multispectral optoacoustic mesoscopy.,Light: Science & Applications

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Spatial heterogeneity of oxygenation and haemodynamics in breast cancer resolved in vivo by conical multispectral optoacoustic mesoscopy.
Light: Science & Applications ( IF 20.6 ) Pub Date : 2020-04-13 , DOI: 10.1038/s41377-020-0295-y
Jiao Li _{1,

2,

3} , Andrei Chekkoury _{2,

3} , Jaya Prakash _{2,

3,

4} , Sarah Glasl _{2,

3} , Paul Vetschera _{2,

3} , Benno Koberstein-Schwarz _{2,

3} , Ivan Olefir _{2,

3} , Vipul Gujrati _{2,

3} , Murad Omar _{2,

3} , Vasilis Ntziachristos _{2,

3}

Affiliation

The characteristics of tumour development and metastasis relate not only to genomic heterogeneity but also to spatial heterogeneity, associated with variations in the intratumoural arrangement of cell populations, vascular morphology and oxygen and nutrient supply. While optical (photonic) microscopy is commonly employed to visualize the tumour microenvironment, it assesses only a few hundred cubic microns of tissue. Therefore, it is not suitable for investigating biological processes at the level of the entire tumour, which can be at least four orders of magnitude larger. In this study, we aimed to extend optical visualization and resolve spatial heterogeneity throughout the entire tumour volume. We developed an optoacoustic (photoacoustic) mesoscope adapted to solid tumour imaging and, in a pilot study, offer the first insights into cancer optical contrast heterogeneity in vivo at an unprecedented resolution of <50 μm throughout the entire tumour mass. Using spectral methods, we resolve unknown patterns of oxygenation, vasculature and perfusion in three types of breast cancer and showcase different levels of structural and functional organization. To our knowledge, these results are the most detailed insights of optical signatures reported throughout entire tumours in vivo, and they position optoacoustic mesoscopy as a unique investigational tool linking microscopic and macroscopic observations.

中文翻译：

圆锥形多光谱光声内镜在体内解决了乳腺癌体内氧合和血流动力学的空间异质性。

肿瘤发展和转移的特征不仅与基因组异质性有关，而且与空间异质性有关，与细胞群的肿瘤内排列，血管形态以及氧气和营养供应有关。尽管通常使用光学（光子）显微镜观察肿瘤的微环境，但它仅能评估数百立方微米的组织。因此，它不适合在整个肿瘤水平上研究生物学过程，整个肿瘤水平至少要大四个数量级。在这项研究中，我们旨在扩展光学可视化并解决整个肿瘤体积中的空间异质性。我们开发了一种适用于实体肿瘤成像的光声（光声）近视镜，并且在一项初步研究中，以前所未有的分辨率<50μm在整个肿瘤块中提供了体内癌症光学对比异质性的第一见解。使用光谱方法，我们解决了三种类型乳腺癌中的未知的氧合作用，脉管系统和灌注模式，并展示了不同水平的结构和功能组织。据我们所知，这些结果是在整个体内整个肿瘤中报告的光学特征的最详细的见解，它们将光声介观法定位为连接微观和宏观观察的独特研究工具。三种类型的乳腺癌的脉管系统和灌注情况，显示出不同水平的结构和功能组织。据我们所知，这些结果是在整个体内整个肿瘤中报告的光学特征的最详细的见解，它们将光声介观法定位为连接微观和宏观观察的独特研究工具。三种类型的乳腺癌的脉管系统和灌注情况，显示出不同水平的结构和功能组织。据我们所知，这些结果是在整个体内整个肿瘤中报告的光学特征的最详细的见解，它们将光声介观法定位为连接微观和宏观观察的独特研究工具。

更新日期：2020-04-24

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