The present study was conducted to characterize the metabolome of accessory gland fluid (AGF) of locally adapted Morada Nova rams, raised in the Brazilian Northeast. AGF was collected by an artificial vagina from five vasectomized rams. Metabolites were identified by gas chromatography-mass spectrometry (GC/MS) and high-performance liquid chromatography-mass spectrometry (LC/MS), with the support of Human Metabolome Database, PubChem, LIPID Metabolites, Pathways Strategy databases, and MetaboAnalyst platforms. There were 182 and 190 metabolites detected by GC/MS and LC/MS, respectively, with an overlap of one molecule. Lipids and lipid-like molecules were the most abundant class of metabolites in the ram AGF (127 compounds), followed by amino acids, peptides, and analogs(103 metabolites). Considering all GC/MS and LC/MS, fructose, glycerol, citric acid, d-mannitol, d-glucose, and l-(+)-lactic acid were the most abundant single metabolites present in the ram AGF. Meaningful pathways associated with AGF metabolites included glycine, serine and threonine metabolism; pantothenate and CoA biosynthesis; galactose metabolism; glutamate metabolism and phenylalanine metabolism, and so forth. In conclusion, the combined use of LC/MS and GC/MS was essential for getting a holistic view of the compounds embedded in the ram AGF. Chemical analysis of the accessory sex gland secretion is relevant for understanding sperm function and fertilization.

中文翻译：

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来自Morada Nova公羊的性腺体液的非靶向代谢组学分析。

进行本研究以表征在巴西东北部生长的局部适应的Morada Nova公羊的副腺体液（AGF）的代谢组。通过人工阴道从五个输精管切开的公羊中收集AGF。在人类代谢物数据库，PubChem，LIPID代谢物，Pathways策略数据库和MetaboAnalyst平台的支持下，通过气相色谱-质谱（GC / MS）和高效液相色谱-质谱（LC / MS）鉴定代谢物。GC / MS和LC / MS分别检测到182个和190个代谢物，其中一个分子重叠。脂质和类脂质分子是ram AGF中最丰富的代谢产物（127种化合物），其次是氨基酸，肽和类似物（103种代谢产物）。考虑到所有GC / MS和LC / MS，果糖，甘油，柠檬酸，d-甘露醇，d-葡萄糖和1-（+）-乳酸是Ram AGF中含量最高的单一代谢产物。与AGF代谢物相关的有意义的途径包括甘氨酸，丝氨酸和苏氨酸的代谢。泛酸和CoA生物合成；半乳糖代谢 谷氨酸代谢和苯丙氨酸代谢，等等。总之，LC / MS和GC / MS的组合使用对于全面了解ram AGF中嵌入的化合物至关重要。辅助性腺分泌物的化学分析与了解精子功能和受精有关。半乳糖代谢 谷氨酸代谢和苯丙氨酸代谢，等等。总而言之，LC / MS和GC / MS的组合使用对于全面了解ram AGF中嵌入的化合物至关重要。附属性腺分泌物的化学分析与了解精子功能和受精有关。半乳糖代谢 谷氨酸代谢和苯丙氨酸代谢，等等。总而言之，LC / MS和GC / MS的组合使用对于全面了解ram AGF中嵌入的化合物至关重要。辅助性腺分泌物的化学分析与了解精子功能和受精有关。