Eleven yeast strains of Yarrowia lipolytica, Metschnikowia sp., Rhodotorula sp. and Rhodosporidium toruloides were grown under nitrogen-limited conditions with crude glycerol employed as substrate in shake flasks, presenting interesting dry cell weight (DCW) production. Three of these strains belonging to Metschnikowia sp. accumulated significant quantities of endopolysaccharides (i.e. the strain V.V.-D4 produced 11.0 g/L of endopolysaccharides, with polysaccharides in DCW ≈ 63% w/w). Six Y. lipolytica strains produced either citric acid or mannitol. Most of the screened yeasts presented somehow elevated lipid and polysaccharides in DCW values at the early steps of growth despite nitrogen appearance in the fermentation medium. Lipid in DCW values decreased as growth proceeded. R. toruloides DSM 4444 cultivated on media presenting higher glycerol concentrations presented interesting lipid-accumulating capacities (maximum lipid = 12.5 g/L, maximum lipid in DCW = 43.0–46.0% w/w, conversion yield on glycerol = 0.16 g/g). Replacement of crude glycerol by xylose resulted in somehow decreased lipid accumulation. In xylose/glycerol mixtures, xylose was more rapidly assimilated from glycerol. R. toruloides total lipids were mainly composed of triacylglycerols. Total cellular fatty acid composition on xylose presented some differences compared with that on glycerol. Cellular lipids contained mainly oleic and palmitic acid.

中文翻译：

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在由生物柴油衍生的粗甘油和甘油/木糖混合物组成的培养基上，在酵母菌株生长过程中产生增值微生物代谢产物。

解脂耶氏酵母，Metschnikowia sp。，Rhodotorula sp。的11个酵母菌株。假单胞菌和拟南芥假单胞菌都在氮限制条件下生长，粗甘油用作摇瓶的底物，产生了令人感兴趣的干细胞重量（DCW）。这些菌株中的三个属于Metschnikowia sp。积累了大量的内多糖（即菌株VV-D4产生11.0 g / L的内多糖，而DCW中的多糖含量约为63％w / w）。六个解脂耶氏酵母菌株产生柠檬酸或甘露醇。尽管在发酵培养基中出现了氮，但大多数筛选的酵母在生长的早期阶段都以某种方式在DCW值上显示出较高的脂质和多糖含量。随着生长的进行，DCW值中的脂质降低。R. 在甘油浓度更高的培养基上培养的类风生物DSM 4444具有有趣的脂质积聚能力（最大脂质= 12.5 g / L，DCW中最大脂质= 43.0–46.0％w / w，甘油转化率= 0.16 g / g）。用木糖替代粗甘油会导致脂质积累减少。在木糖/甘油混合物中，木糖与甘油的吸收更快。R. toruloides总脂质主要由三酰基甘油组成。与甘油相比，木糖上的总细胞脂肪酸组成存在一些差异。细胞脂质主要包含油酸和棕榈酸。用木糖替代粗甘油会导致脂质积累减少。在木糖/甘油混合物中，木糖与甘油的吸收更快。R. toruloides总脂质主要由三酰基甘油组成。与甘油相比，木糖上的总细胞脂肪酸组成存在一些差异。细胞脂质主要包含油酸和棕榈酸。用木糖替代粗甘油会导致脂质积累减少。在木糖/甘油混合物中，木糖与甘油的吸收更快。R. toruloides总脂质主要由三酰基甘油组成。与甘油相比，木糖上的总细胞脂肪酸组成存在一些差异。细胞脂质主要包含油酸和棕榈酸。