Lactic acid bacteria (LAB) can be used to increase the folate in foods by in situ fortification. Seventy LAB were screened for their ability to produce folate during growth in de Man, Rogosa and Sharpe/M17 broth. Lactobacillus casei, Lactobacillus plantarum, Lactobacillus paracasei subsp. paracasei, Lactobacillus rhamnosus, Lactobacillus delbrueckii subsp. bulgaricus, Streptococcus thermophilus, Lactococcus lactis subsp. lactis, Enterococcus faecium and Enterococcus lactis were able to synthetize folates in the medium, even if to a different extent. The 47 folate-producing strains were further analyzed by microbiological assay, for total, extra and intracellular folate. Enterococcus faecium VC223 and E. lactis BT161 were able to produce in cultural medium 123,625.74 ± 8.00 ng/ml and 384.22 ± 5.00 ng/ml of folate, respectively. Five strains were further examined for their ability to synthesize folate in cheese. The folate content increased with ripening up to by 54% after 30 d when L. casei VC199 was used and up to 108% and 113% after 60 d, with L. paracasei SE160 and E. lactis BT161 respectively exceeding 100 ng/100g. Results encourage the use of specific LAB to obtain natural folate bio-enriched dairy products improving folate intake.

中文翻译：

---

筛选产生叶酸的乳酸菌及其作为干酪生物富集辅助培养物的潜在用途。

乳酸菌（LAB）可用于通过原位强化来增加食品中的叶酸。筛选了70个LAB在de Man，Rogosa和Sharpe / M17肉汤生长过程中产生叶酸的能力。干酪乳杆菌，植物乳杆菌，副干酪乳杆菌亚种。副干酪，鼠李糖乳杆菌，德氏乳杆菌亚种。保加利亚，嗜热链球菌，乳酸乳球菌亚种。乳酸菌，粪肠球菌和乳酸肠球菌即使在不同程度上也能在培养基中合成叶酸。通过微生物测定进一步分析了47种产生叶酸的菌株的总，额外和细胞内叶酸。粪肠球菌VC223和乳杆菌BT161能够分别在培养基中产生123,625.74±8.00ng / ml和384.22±5.00ng / ml的叶酸。进一步检查了五种菌株在奶酪中合成叶酸的能力。当使用干酪乳杆菌VC199时，叶酸含量随着成熟而增加，在30 d后高达54％，在60 d后高达108％和113％，其中副干酪乳杆菌SE160和乳杆菌BT161分别超过100 ng / 100g。结果鼓励使用特定的LAB获得富含叶酸的生物富集乳制品，从而改善叶酸的摄入量。