This study aimed to compare the in vitro effects of chitosan oligomers and polymer with commonly-used plant growth regulators (PGRs) on seed germination, protocorm formation, and organ development in Serapias vomeracea. The effects of N-acetylated (10%) chitosan oligomer mixture (CHI-OM) with a degree of polymerization (DP) between 2 and 15 (5, 10, 15, and 20 mg L⁻¹) and chitosan polymer (CHI-P) with a DP of 70 were compared with commonly-used cytokinins [6-benzylaminopurine (BAP) and kinetin (KIN)], auxins [indole-3-acetic acid (IAA) and indole-3-butyric acid (IBA)], and jasmonic acid (JAS) at 0.25, 0.5, 1.0, and 2.0 mg L⁻¹. The medium supplemented with CHI-P at 5 mg L⁻¹ gave the highest seed germination rate, whereas JAS and CHI-OM triggered protocorm formation better than the all treatments tested. The JAS and IAA treatments resulted in intense browning of the roots. The CHI-P treatments at high concentrations and JAS treatments at moderate concentrations increased mean shoot length while the medium containing KIN at 0.5 mg L⁻¹ induced root elongation significantly. The root elongation-inhibitory effect of chitosan was observed at the higher concentrations of CHI-OM, but the media containing 10 mg L⁻¹ CHI-OM and 15 mg L⁻¹ CHI-P triggered adventitious rooting. However, the highest tuberization success was found after 10 mg L⁻¹ CHI-OM and 0.5 mg L⁻¹ JAS treatments. Tuber development was achieved in the media supplemented with BAP and CHI-OM. This study suggested that well-characterized chitosan could be used as an alternative to JAS and BAP in orchid cultures.

这项研究的目的是比较壳聚糖低聚物和聚合物与常用植物生长调节剂（PGRs）对Serapias vomeracea种子萌发，原球茎形成和器官发育的体外影响。的效果Ñ 2和15（5，10，15，和20毫克的L之间-acetylated（10％）脱乙酰壳多糖低聚物混合物（CHI-OM）聚合度（DP）的^-1）和脱乙酰壳多糖聚合物（罗致光P）具有70的DP与常用的细胞分裂素[6-苄氨基嘌呤（BAP）和激动素（KIN）]，生长素[吲哚-3-乙酸（IAA）和吲哚-3-丁酸（IBA）]进行了比较，茉莉酸（JAS）的浓度分别为0.25、0.5、1.0和2.0 mg L ^-1。补充CHI-P 5 mg L ^-1的培养基种子发芽率最高，而JAS和CHI-OM引发的原球茎形成要优于所有测试方法。JAS和IAA处理导致根部强烈褐变。高浓度的CHI-P处理和中等浓度的JAS处理均增加了平均芽长，而KIN含量为0.5 mg L ^-1的培养基显着诱导了根伸长。在较高浓度的CHI-OM中观察到了壳聚糖的根伸长抑制作用，但是含有10 mg L ^-1 CHI-OM和15 mg L ^-1 CHI-P的培养基引发了不定根。但是，在10 mg L ^-1 CHI-OM和0.5 mg L ^-1后发现了最高的块茎成功率JAS治疗。在补充了BAP和CHI-OM的培养基中达到了块茎发育。这项研究表明，在兰花文化中，表征良好的壳聚糖可以替代JAS和BAP。