Centaurium erythraea Rafn. (common centaury, Gentianaceae) is a medicinal plant with great regeneration potential and developmental plasticity in vitro. Centaury can be regenerated from leaf explants by both somatic embryogenesis (SE) and shoot development (SD). We believe that its regeneration potential and developmental plasticity rest on high activity of certain genes, which may not be active or present in species recalcitrant to in vitro regeneration. However, there are no sequenced Gentianaceae genomes to support investigation of the molecular events during SE or SD. To this end, we have sequenced six centaury transcriptomes (embryogenic calli, globular somatic embryos, cotyledonary somatic embryos, adventitious buds, leaves and roots of in vitro grown plants) and de novo assembled centaury referent transcriptome comprising 105.726 genes. The high quality and completeness transcriptome was functionally annotated against NCBI nt, Swissprot and PFAM databases with KOG and GO enrichment. In addition, 11 housekeeping and functional genes were validated for expression stability in 27 tissue samples representing the processes of SE and SD, plants from nature and wounded tissues using GeNorm and NormFinder. The most stable genes that can be used for expression studies during SE, SD and in vitro manipulations are Ribosomal protein L2 (RPL2) and TATA binding protein 1 (TBP1) in combination with RAS (Rat Sarcoma)-related Nuclear protein (RAN) or Adenosine kinase (AK). These results comprise a complete framework for the search for genes involved in SE and SD, but may also be useful in identifying genes involved in biosynthesis of C. erythraea secondary metabolites.

Centaurium erythraea Rafn。（普通cent科，龙胆科）是具有巨大再生潜力和体外发育可塑性的药用植物。可以通过体细胞胚发生（SE）和枝条发育（SD）从叶片外植体中再生百叶。我们相信其再生潜力和发育可塑性取决于某些基因的高活性，这些基因可能不活跃或不存在于体外再生的顽固物种中。但是，没有测序龙胆科基因组以支持对SE或SD期间分子事件的研究。为此，我们已对六个百分百转录组进行了测序（胚发生的愈伤组织，球状体细胞胚，子叶体细胞胚，不定芽，离体芽，离体生长的植物的根和叶）和从头开始的包含105.726个基因的百分百百年组参照转录组。高质量和完整的转录组通过带有KOG和GO富集的NCBI nt，Swissprot和PFAM数据库进行了功能注释。此外，使用GeNorm和NormFinder对11个持家基因和功能基因在代表SE和SD进程，自然界植物和受伤组织的27个组织样品中的表达稳定性进行了验证。SE期间可用于表达研究的最稳定的基因，SD和体外操作是核糖体蛋白L2（RPL2）和TATA结合蛋白1（TBP1）与RAS（大鼠肉瘤）相关的核蛋白（RAN）或腺苷激酶（AK）结合使用。这些结果为寻找SE和SD涉及的基因提供了一个完整的框架，但也可能有助于鉴定与SE和SD的生物合成有关的基因。C.红斑次生代谢产物。