Satureja khuzistanica contains approximately 3% essential oil, and carvacrol is its major constituent. This research aimed to evaluate effect of Sodium pyrophosphate (NaPP) on secondary metabolites, antioxidant, antimicrobial, and cytotoxicity properties of S. khuzistanica. In this regard, the microshoots prepared from this plant were cultured in Linsmaier and Skoog supplemented with different concentrations of NaPP (0, 0.1, 0.2 and 0.5 mM) and have been incubated for 25 days. Results showed that NaPP not only increased the growth of S. khuzistanica but also enhanced its chlorophyll, carotenoids, phenol, and flavonoid. Furthermore, carvacrol and 1-deoxy-dxylulose 5-phosphate reductoisomerase in NaPP-treated plantlets, as compared to the controls, were found three- and two-fold increases, respectively; this highlights activity of MEP pathway responsible for biosynthesis of carvacrol. By affecting secondary metabolites, NaPP improved some properties of S. khuzistanica such as antimicrobial (against periodontal bacteria), antioxidant, and cytotoxicity. In general, our results revealed that NaPP, exclusively at 0.2 mM, can accumulate different bioactive compounds in S. khuzistanica resulting in enhancing its pharmacological and medicinal properties.

Satureja khuzistanica含有约3％的精油，香芹酚是其主要成分。这项研究旨在评估焦磷酸钠（NaPP）对苦参的次生代谢产物，抗氧化剂，抗微生物剂和细胞毒性的影响。在这方面，将从该植物制备的微芽在补充有不同浓度的NaPP（0、0.1、0.2和0.5mM）的Linsmaier和Skoog中培养，并孵育了25天。结果表明NaPP不仅增加了苦参的生长而且还增强了其叶绿素，类胡萝卜素，苯酚和类黄酮的含量。此外，与对照相比，NaPP处理的幼苗中的香芹酚和1-脱氧-二甲苯基葡萄糖5-磷酸还原异构酶分别增加了三倍和两倍。这突出了负责香芹酚生物合成的MEP途径的活性。通过影响次生代谢产物，NAPP改进的一些性质S. khuzistanica例如抗微生物（抗牙周病菌），抗氧化剂，和细胞毒性。通常，我们的结果表明，NaPP仅以0.2 mM可以在苦参中积累不同的生物活性化合物，从而增强其药理和药用性能。