Photomixotrophic micropropagation systems have been associated with improved development of several plant species. In this work, we established an efficient protocol for the in vitro micropropagation of Bambusa oldhamii Munro by studying the influence of the rainy and dry seasons on plant tissue contamination, the effects of the gaseous atmosphere inside the culture flasks, and the effects of radiation conditions on plant morphogenetic events. Explants were collected from March to December 2016. The conditions consisted of 100% blue (455 nm), 100% red (630 nm), 30% blue + 70% red, and 30% red + 70% blue LED light or fluorescent light only (as a control). For induction of axillary sprouting, the explants were inoculated in MS media + 2.27 µM thidiazuron or 3.40 µM paclobutrazol. The best growth responses were observed for material collected from June to July. There was an increase of 81.79% in the number of leaves under the 30% blue + 70% red LED treatment in comparison to the control. The facilitation of gas exchange resulted in improved shoot production, with an increase of 70.80% under 30% red + 70% blue LED light in comparison to that of treatments without gas exchange. The blue LED light had an important photomorphogenic effect on the development of bamboo explants into shoots, as shoot proliferation increased. The results obtained will aid in the development of protocols for the in vitro culture of other species of bamboo as well as for the establishment of an efficient protocol to address rooting for this and other bamboo species.

光合营养微繁殖系统已经与几种植物物种的改良发育相关。在这项工作中，我们建立了Bambusa oldhamii的体外微繁殖的有效方案Munro通过研究雨季和旱季对植物组织污染的影响，培养瓶内气体气氛的影响以及辐射条件对植物形态发生事件的影响来研究Munro。从2016年3月至2016年12月收集了外植体。条件包括100％蓝色（455 nm），100％红色（630 nm），30％蓝色+ 70％红色和30％红色+ 70％蓝色LED灯或荧光灯仅（作为控件）。为了诱导腋芽，将外植体接种在MS培养基+ 2.27 µM噻唑隆或3.40 µM多效唑中。从六月到七月收集的材料观察到最佳的生长响应。与对照相比，在30％蓝色+ 70％红色LED处理下，叶片数量增加了81.79％。气体交换的便利性提高了芽的产量，与不使用气体交换的处理相比，在30％红色+ 70％蓝色LED灯下的光输出增加了70.80％。随着竹笋增殖的增加，蓝色LED灯对竹外植体发展成竹笋具有重要的光致形态作用。获得的结果将有助于开发其他竹子的体外培养方法的协议，以及建立解决该竹子和其他竹子生根问题的有效协议。