Although various types of artificial skeletal muscle tissue have been reported, the contractile forces generated by tissue-engineered artificial skeletal muscles remain to be improved for biological model and clinical applications. In this study, we investigated the effects of extracellular matrix (ECM) and supplementation of a small molecule, which has been reported to enhance α7β1 integrin expression (SU9516), on cell migration speed, cell fusion rate, myoblast (mouse C2C12 cells) differentiation and contractile force generation of tissue-engineered artificial skeletal muscles. When cells were cultured on varying ECM coated-surfaces, we observed significant enhancement in the migration speed, while the myotube formation (differentiation ratio) decreased in all except for cells cultured on Matrigel coated-surfaces. In contrast, SU9516 supplementation resulted in an increase in both the myotube width and differentiation ratio. Following combined culture with a Matrigel-coated surface and SU9516 supplementation, myotube width was further increased. Additionally, contractile forces produced by the tissue-engineered artificial skeletal muscles was augmented following combined culture. These findings indicate that regulation of the cell-ECM interaction is a promising approach to improve the function of tissue-engineered artificial skeletal muscles.

尽管已经报道了各种类型的人造骨骼肌组织，但是对于生物学模型和临床应用，由组织工程化的人造骨骼肌产生的收缩力仍有待改善。在这项研究中，我们调查了细胞外基质（ECM）和补充小分子（据报道可增强α7β1整联蛋白表达（SU9516））对细胞迁移速度，细胞融合率，成肌细胞（小鼠C2C12细胞）分化的影响组织工程化的人造骨骼肌的收缩力和收缩力的产生。当在不同的ECM涂层表面上培养细胞时，我们观察到迁移速度显着增强，而除在Matrigel涂层表面上培养的细胞外，所有肌管的形成（分化率）均下降。相反，SU9516的添加导致肌管宽度和分化率的增加。在结合了基质胶包被的表面和SU9516的补充培养后，肌管宽度进一步增加。此外，组合培养后，由组织工程化的人造骨骼肌产生的收缩力增加。这些发现表明，调节细胞-ECM相互作用是改善组织工程人造骨骼肌功能的有前途的方法。联合培养后，由组织工程化的人造骨骼肌产生的收缩力增加。这些发现表明，调节细胞-ECM相互作用是改善组织工程人造骨骼肌功能的有前途的方法。联合培养后，由组织工程化的人造骨骼肌产生的收缩力增加。这些发现表明，调节细胞-ECM相互作用是改善组织工程人造骨骼肌功能的有前途的方法。